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A 15 aa C terminal peptide from rat AQP2.
Our Abpromise guarantee covers the use of ab15081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 29 kDa.|
|IHC-P||Use a concentration of 2 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ELISA||Use a concentration of 0.5 - 1 µg/ml.|
ab15081 staining Aquaporin 2 in rat kidney tissue section by IHC(P). Tissue underwent fixation in formalin, embedded in paraffin, heat mediated antigen retrieval in 10mM EDTA buffer pH 8.0 and blocking in 5% serum for 20 minutes at 20°C. The primary antibody, diluted 1/300 in TBSTween and incubated with sample for 1 hour at 20°C. A HRP conjugated goat polyclonal to rabbit was used undiluted as the secondary.