Overview

  • Product nameAnti-Aquaporin 2 antibodySee all Aquaporin 2 primary antibodies ...
  • Description
    Rabbit polyclonal to Aquaporin 2
  • Tested applicationsWB, IHC-P, ELISAmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    A 15 aa C terminal peptide from rat AQP2.

  • Positive control
    • Rat and human kidney cortex, apical membranes, inner and outer medulla (postranslational or glycosylation).
  • General notesIn kidneys the AQP2 protein is mainly expressed in collecting ducts, which plays an important role in determining the final urinary output of water and solutes. The tissue section of this part will be a good positive control.

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: 40% Glycerol, 0.1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab15081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 2 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ELISA Use a concentration of 0.5 - 1 µg/ml.

Target

  • FunctionForms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificityExpressed in renal collecting tubules.
  • Involvement in diseaseDefects in AQP2 are the cause of diabetes insipidus nephrogenic autosomal (ANDI) [MIM:125800]; also known as diabetes insipidus nephrogenic type 2. ANDI is caused by the inability of the renal collecting ducts to absorb water in response to arginine vasopressin. It is characterized by excessive water drinking (polydypsia), excessive urine excretion (polyuria), persistent hypotonic urine, and hypokalemia. Inheritance can be autosomal dominant or recessive.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • Cellular localizationApical cell membrane. Cytoplasmic vesicle membrane. Shuttles from vesicles to the apical membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

Anti-Aquaporin 2 antibody images

  • ab15081 staining Aquaporin 2 in rat kidney tissue section by IHC(P). Tissue underwent fixation in formalin, embedded in paraffin, heat mediated antigen retrieval in 10mM EDTA buffer pH 8.0 and blocking in 5% serum for 20 minutes at 20°C. The primary antibody, diluted 1/300 in TBSTween and incubated with sample for 1 hour at 20°C. A HRP conjugated goat polyclonal to rabbit was used undiluted as the secondary.  

    See Abreview

  • ab15081at a 1/250 dilution staining Aquaporin 2 in human kidney tissue by Immunohistochemistry (formalin fixed, paraffin embedded sections), incubated for 20 minutes at 25°C. Heat mediated retrieval step performed using EDTA at 100°C for 20 minutes. Secondary used undiluted monoclonal Goat anti-rabbit, Leica Refine, HRP polymer.

    See Abreview

References for Anti-Aquaporin 2 antibody (ab15081)

This product has been referenced in:
  • Airik R  et al. Renal-Retinal Ciliopathy Gene Sdccag8 Regulates DNA Damage Response Signaling. J Am Soc Nephrol N/A:N/A (2014). Human . Read more (PubMed: 24722439) »
  • Thoroddsen A  et al. The Water Permeability Channels Aquaporins 1-4 Are Differentially Expressed in Granulosa and Theca Cells of the Preovulatory Follicle during Precise Stages of Human Ovulation. J Clin Endocrinol Metab 96:1021-8 (2011). IHC-P ; Human . Read more (PubMed: 21252246) »

See all 5 Publications for this product

Product Wall

Thank you for your email. Please find below the tetsing discount code for ab85379. At the bottom of the email, I have listed the information regarding the free of charge replacement for ab15081 and ab58968.
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Thank you for your reply and the additional information. This is very helpful to us for our internal
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Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Kidney)
Specification Kidney
Fixative 10% NBF
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: TRIS/EDTA pH 9.0
Permeabilization No
Blocking step Serum Free Protein Block as blocking agent for 5 minute(s) · Concentration: 0.25%
Username

Ms. Elizabeth Chlipala

Verified customer

Submitted Aug 20 2010

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: EDTA, 100C, 20 minutes
Permeabilization No
Username

Abcam user community

Verified customer

Submitted Dec 02 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Kidney)
Specification Kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: pH 9,0
Permeabilization No
Blocking step Peroxidase Blocking solution (Dako) as blocking agent for 5 minute(s) · Concentration: 3% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 06 2009

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (kidney)
Specification kidney
Fixative Formaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10mM EDTA pH8.0
Permeabilization No
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Nov 06 2008

Thank you for getting back to me. The approach that you are employing is one that I would recommend and very similar to the approach that was used to test this antibody. We often find that some of our antibodies require a degree of optimisation as ...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. I am in the process of contacting the lab with respect to the validation that would have been performed on the batch that you have been having...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"