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Synthetic non-phosphopeptide derived from human Aquaporin 2 around the phosphorylation site of serine 256
Our Abpromise guarantee covers the use of ab62628 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||1/500 - 1/1000.|
ab62628 staining Aquaporin in Mouse embryonic kidney tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100 and blocked with 1% BSA for 1 hour at 20°C. Samples were incubated with primary antibody (1/500 in blocking solution) for 1 hour at 20°C. An Alexa Fluor® 568-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
Left image untreated.
Right image treated with peptide.