Anti-Aquaporin 2 (phospho S256) antibody (ab111346)

Overview

  • Product nameAnti-Aquaporin 2 (phospho S256) antibody
    See all Aquaporin 2 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 2 (phospho S256)
  • Specificityab111346 detects endogenous levels of Aquaporin 2 only when phosphorylated at serine 256.
  • Tested applicationsSuitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic phosphopeptide phosphopeptide derived from Human Aquaporin 2 around the phosphorylation site of serine 256 (R-Q-SP-V-E).

  • Positive control
    • Human brain tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituents: 49.1% PBS, 50% Glycerol, 0.88% Sodium chloride
    Note: PBS (without Mg2+ and Ca2+)
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesab111346 was affinity-purified from Rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab111346 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionForms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificityExpressed in renal collecting tubules.
  • Involvement in diseaseDiabetes insipidus, nephrogenic, autosomal
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • Cellular localizationApical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

Anti-Aquaporin 2 (phospho S256) antibody images

  • ab111346 at 1/50 dilution staining Aquaporin 2 in Paraffin-embedded Human Brain tissue by Immunohistochemistry. The image on the right is treated with the synthesized phosphopeptide.

References for Anti-Aquaporin 2 (phospho S256) antibody (ab111346)

ab111346 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (IMCD Cells)
Gel Running Conditions Reduced Denaturing (12 %)
Loading amount 25 µg
Treatment - and + 100 nM AVP
Specification IMCD Cells
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Username

Mrs. Andrea Geelhaar

Verified customer

Submitted Feb 22 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"