Overview

  • Product name
    Anti-Aquaporin 2 (phospho S261) antibody
    See all Aquaporin 2 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 2 (phospho S261)
  • Specificity
    This antibody is specific for ~29k Aquaporin 2 protein phosphorylated at Ser261. It also recognizes the glycosylated form of Aquaporin 2 at ~ 37k. Immunolabeling of the Aquaporin 2 band is blocked by preadsorption with the phospho peptide used as antigen but not by the corresponding dephospho peptide.
  • Tested applications
    Suitable for: WB, Flow Cytmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Chicken, Cow, Dog, Human, Non human primates
  • Immunogen

    Phosphopeptide corresponding to amino acid residues surrounding the phospho Serine 261 of rat Aquaporin 2.

  • Positive control
    • Rat kidney lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 50% Glycerol, 0.1mg/ml BSA, 150mM Sodium chloride, 10mM HEPES, pH 7.5
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab64154 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).
Flow Cyt Use at an assay dependent concentration. PubMed: 20826573ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

Target

  • Function
    Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient.
  • Tissue specificity
    Expressed in renal collecting tubules.
  • Involvement in disease
    Diabetes insipidus, nephrogenic, autosomal
  • Sequence similarities
    Belongs to the MIP/aquaporin (TC 1.A.8) family.
  • Domain
    Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    modifications
    Ser-256 phosphorylation is necessary and sufficient for expression at the apical membrane. Endocytosis is not phosphorylation-dependent.
  • Cellular localization
    Apical cell membrane. Basolateral cell membrane. Cytoplasmic vesicle membrane. Golgi apparatus, trans-Golgi network membrane. Shuttles from vesicles to the apical membrane. Vasopressin-regulated phosphorylation is required for translocation to the apical cell membrane. PLEKHA8/FAPP2 is required to transport AQP2 from the TGN to sites where AQP2 is phosphorylated.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADH water channel antibody
    • AQP 2 antibody
    • AQP CD antibody
    • AQP-2 antibody
    • AQP-CD antibody
    • AQP2 antibody
    • AQP2_HUMAN antibody
    • AQPCD antibody
    • Aquaporin 2 collecting duct antibody
    • Aquaporin CD antibody
    • Aquaporin-2 antibody
    • Aquaporin-CD antibody
    • Aquaporin2 antibody
    • Aquaporine 2 antibody
    • Collecting duct water channel protein antibody
    • MGC34501 antibody
    • Water channel aquaporin 2 antibody
    • Water channel protein for renal collecting duct antibody
    • WCH CD antibody
    • WCH-CD antibody
    • WCHCD antibody
    see all

Images

  • All lanes : Anti-Aquaporin 2 (phospho S261) antibody (ab64154) at 1/1000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat kidney lysate, with blocking peptide


    Predicted band size : 29 kDa
    Observed band size : 29,37 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Kelley R  et al. Tubular cell-enriched subpopulation of primary renal cells improves survival and augments kidney function in rodent model of chronic kidney disease. Am J Physiol Renal Physiol 299:F1026-39 (2010). Flow Cyt ; Rat . Read more (PubMed: 20826573) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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