Overview

  • Product nameAnti-Aquaporin 3 antibody
    See all Aquaporin 3 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 3
  • Tested applicationsWB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse
  • Immunogen

    Synthetic peptide:

    N-CHLEQPPPSTEAENVKLAHMKHKEQI

    , corresponding to C terminal amino acids 267-292 of Rat Aquaporin 3 (NP_113891.1).

  • Positive control
    • Rat kidney tissue; Rat kidney lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human Brain Hippocampus (Alzheimer's).

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab125045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/4000. Predicted molecular weight: 31 kDa.
ICC/IF 1/200 - 1/400.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Anti-Aquaporin 3 antibody images

  • IHC image of Aquaporin 3 staining in Human Hippocampus (Alzheimer's) formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125045, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab125045, at 1/200 dilution, staining Aquaporin 3 in Rat kidney tissues by Immunofluorescence.
  • All lanes : Anti-Aquaporin 3 antibody (ab125045) at 1/2000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat kidney lysate


    Predicted band size : 31 kDa

References for Anti-Aquaporin 3 antibody (ab125045)

ab125045 has not yet been referenced specifically in any publications.

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