Overview

  • Product nameAnti-Aquaporin 3 antibody
    See all Aquaporin 3 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 3
  • Tested applicationsWB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Rabbit, Horse
  • Immunogen

    Synthetic peptide:

    N-CHLEQPPPSTEAENVKLAHMKHKEQI

    , corresponding to C terminal amino acids 267-292 of Rat Aquaporin 3 (NP_113891.1).

  • Positive control
    • Rat kidney tissue; Rat kidney lysate This antibody gave a positive result in IHC in the following FFPE tissue: Human Brain Hippocampus (Alzheimer's).

Properties

Applications

Our Abpromise guarantee covers the use of ab125045 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/4000. Predicted molecular weight: 31 kDa.
ICC/IF 1/200 - 1/400.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • FunctionWater channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
  • Tissue specificityWidely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localizationBasolateral cell membrane. In collecting ducts of kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 3 antibody
    • AQP-3 antibody
    • Aqp3 antibody
    • AQP3_HUMAN antibody
    • Aquaglyceroporin-3 antibody
    • Aquaporin 3 (GIL blood group) antibody
    • Aquaporin 3 (Gill blood group) antibody
    • Aquaporin-3 antibody
    • Aquaporin3 antibody
    • GIL antibody
    • Gill blood group antibody
    see all

Anti-Aquaporin 3 antibody images

  • IHC image of Aquaporin 3 staining in Human Hippocampus (Alzheimer's) formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab125045, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ab125045, at 1/200 dilution, staining Aquaporin 3 in Rat kidney tissues by Immunofluorescence.
  • All lanes : Anti-Aquaporin 3 antibody (ab125045) at 1/2000 dilution

    Lane 1 : Rat kidney lysate
    Lane 2 : Rat kidney lysate
    Lane 3 : Rat kidney lysate
    Lane 4 : Rat kidney lysate


    Predicted band size : 31 kDa

References for Anti-Aquaporin 3 antibody (ab125045)

ab125045 has not yet been referenced specifically in any publications.

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