• Product nameAnti-Aquaporin 3 antibody
    See all Aquaporin 3 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 3
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide from the C terminal region of Human Aquaporin 3.

  • Positive control
    • WB: Rat kidney, mouse lung and mouse kidney tissue lysates. IHC-P: Rat kidney tissue. ICC/IF: formaldehyde fixed MCF-7 cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage bufferPreservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 0.1% Dibasic monohydrogen sodium phosphate, 0.45% Sodium chloride, 2.5% BSA
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab125219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.Can be blocked with Aquaporin 3 peptide (ab195690). The detection limit of ab125219 is approximately 1ng/lane under non-reducing and reducing conditions.
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 µg/ml.
IP Use a concentration of 5 µg/ml.
Flow Cyt Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.


  • FunctionWater channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
  • Tissue specificityWidely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localizationBasolateral cell membrane. In collecting ducts of kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 3 antibody
    • AQP-3 antibody
    • Aqp3 antibody
    • AQP3_HUMAN antibody
    • Aquaglyceroporin-3 antibody
    • Aquaporin 3 (GIL blood group) antibody
    • Aquaporin 3 (Gill blood group) antibody
    • Aquaporin-3 antibody
    • Aquaporin3 antibody
    • GIL antibody
    • Gill blood group antibody
    see all

Anti-Aquaporin 3 antibody images

  • All lanes : Anti-Aquaporin 3 antibody (ab125219) at 1 µg/ml

    Lane 1 : Rat kidney tissue lysate
    Lane 2 : Mouse lung tissue lysate
    Lane 3 : Mouse kidney tissue lysate

    Predicted band size : 31 kDa
  • ab125219 at 1µg/ml staining Aquaporin 3 in Paraaffin-embedded Rat kidney tissue by Immunohistochemistry.
  • Aquaporin 3 was immunoprecipitated using 0.5mg Mouse Kidney extract, 5µg of Rabbit polyclonal to Aquaporin 3 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Mouse Kidney extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab125219.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 31kDa: Aquaporin 3.
  • ICC/IF image of ab125219 stained MCF-7 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab125219 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Aquaporin 3 antibody (ab125219)

This product has been referenced in:
  • Nazemi S  et al. Reciprocity in the developmental regulation of aquaporins 1, 3 and 5 during pregnancy and lactation in the rat. PLoS One 9:e106809 (2014). WB ; Rat . Read more (PubMed: 25184686) »

See 1 Publication for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization No
Sample Human Cell (Human bronchial epithelial cells)
Specification Human bronchial epithelial cells
Gating Strategy Single cells (gated on FSC-H vs FSC-W), debris excluded (gated on FSC-A vs SSC-A) and live cells (gated on live/dead stain negative population)
Preparation Cell harvesting/tissue preparation method: Trypsinisation
Sample buffer: 10% DMEM

Abcam user community

Verified customer

Submitted Apr 15 2014