Overview

  • Product nameAnti-Aquaporin 3 antibody
    See all Aquaporin 3 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 3
  • Tested applicationsSuitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with: Rat
    Predicted to work with: Mouse, Human
  • Immunogen

    Synthetic peptide (Rat) conjugated to KLH (C terminal).

  • Positive control
    • Rat kidney medulla or outer cortex.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab15117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 32 kDa.Can be blocked with Aquaporin 3 peptide (ab80550).
ELISA 1/10000 - 1/50000.
IHC-Fr 1/50.

Target

  • FunctionWater channel required to promote glycerol permeability and water transport across cell membranes. Acts as a glycerol transporter in skin and plays an important role in regulating SC (stratum corneum) and epidermal glycerol content. Involved in skin hydration, wound healing, and tumorigenesis. Provides kidney medullary collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Slightly permeable to urea and may function as a water and urea exit mechanism in antidiuresis in collecting duct cells. It may play an important role in gastrointestinal tract water transport and in glycerol metabolism.
  • Tissue specificityWidely expressed in epithelial cells of kidney (collecting ducts) and airways, in keratinocytes, immature dendritic cells and erythrocytes. Isoform 2 is not detectable in erythrocytes at the protein level.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localizationBasolateral cell membrane. In collecting ducts of kidney.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 3 antibody
    • AQP-3 antibody
    • Aqp3 antibody
    • AQP3_HUMAN antibody
    • Aquaglyceroporin-3 antibody
    • Aquaporin 3 (GIL blood group) antibody
    • Aquaporin 3 (Gill blood group) antibody
    • Aquaporin-3 antibody
    • Aquaporin3 antibody
    • GIL antibody
    • Gill blood group antibody
    see all

References for Anti-Aquaporin 3 antibody (ab15117)

This product has been referenced in:
  • Ratovitski EA Phospho-?Np63a regulates AQP3, ALOX12B, CASP14 and CLDN1 expression through transcription and microRNA modulation. FEBS Lett 587:3581-6 (2013). Read more (PubMed: 24070899) »

See 1 Publication for this product

Product Wall

Thank you very much for your interest in ab15117.

The C-terminal immunogen is predicted to be a cytoplasmic domain, not extracellular, based on information from the supplying lab. It may be suitable for FACS, but this application is untested....

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Thank you for submitting the Abreviews with ab9871, ab15117, ab110534 and ab18499 using dot blot. The results look really good.

I will be submitting your reviews manually. In order to issue the Abpoints generated to the relevant account, cou...

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Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (Kidney)
Specification Kidney
Fixative Acetone
Permeabilization No
Blocking step BSA as blocking agent for 10 minute(s) · Concentration: 0.5% · Temperature: 20°C
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Verified customer

Submitted Jan 12 2010

Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Intestine)
Specification Intestine
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 26°C
Username

Abcam user community

Verified customer

Submitted Aug 29 2007

Thank you for your enquiry. This antibody has not been tested in these applications. We mistakenly had these applications on the datasheet but removed them on April 5, 2006. The antibody may work in these applications, but we have not tested it. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"