• Product nameAnti-Aquaporin 4 antibody [4/18]
    See all Aquaporin 4 primary antibodies
  • Description
    Mouse monoclonal [4/18] to Aquaporin 4
  • Tested applicationsSuitable for: ELISA, IHC-Fr, IHC-P, WB, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Rabbit, Human, Zebrafish
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide:


    , corresponding to amino acids 301-318 of Aquaporin 4

  • EpitopeIntracellular C-terminal AQP 4 epitope


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, 1mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number4/18
  • IsotypeIgG3
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab9512 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. PubMed: 11595449
IHC-Fr Use at an assay dependent concentration.
IHC-P 1/100. Perform heat mediated antigen retrieval via the microwave method before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. PubMed: 19831719
IHC-FoFr Use at an assay dependent concentration. PubMed: 22438975


  • FunctionForms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • Tissue specificityBrain - muscle >> heart, kidney, lung, and trachea.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Anti-Aquaporin 4 antibody [4/18] images

  • Immunohistochemical staining of aquaporin 4 in rat brain.
  • Immunohistochemical analysis of mouse brain tissue, staining Aquaporin 4 with ab9512 at 1/100 dilution. Staining was detected using a biotin-conjugated anti-mouse antibody followed by FITC-conjugated streptavidin.

References for Anti-Aquaporin 4 antibody [4/18] (ab9512)

This product has been referenced in:
  • Leonard AV  et al. NK1 receptor blockade is ineffective in improving outcome following a balloon compression model of spinal cord injury. PLoS One 9:e98364 (2014). IHC-P ; Rabbit . Read more (PubMed: 24859234) »
  • Glover LE  et al. Immediate, but not delayed, microsurgical skull reconstruction exacerbates brain damage in experimental traumatic brain injury model. PLoS One 7:e33646 (2012). IHC-FoFr ; Rat . Read more (PubMed: 22438975) »

See all 6 Publications for this product

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (intervertebral Disc)
Antigen retrieval step None
Permeabilization No
Specification intervertebral Disc
Blocking step 1% BSA in 75% TBS 35% Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
Fixative 10% Neutral Buffered Formalin

Abcam user community

Verified customer

Submitted Jul 14 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Rat Tissue sections (Brain)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Ph9 Microwave
Permeabilization No
Specification Brain
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1500µg/mL · Temperature: RT°C
Fixative Paraformaldehyde

Gaia Brezzo

Verified customer

Submitted Sep 06 2016

Thank you for this information. It is unusual for one to see a band and then subsequently see nothing, which is why I thought you may have been stripping and re-probing. You may improve your result by increasing the concentration of the primary antibod...

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I am sorry to hear that this antibody is not providing satisfactory results.
You indicated that the secondary antibody used is a rabbit antibody that reacts with rat, however the primary is in mouse so you would need an anti-mouse antibody. Please ...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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Thank you for contacting us.

We realize that our products can be considered to have premium price point. However, we feel that the product and service which we offer are of the highest possible quality. Under our Abpromise guarantee, ourprod...

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Thank you for contacting us. I am glad to hear that this replacement product is performing better than the last product.

Many factors can alter the predicted molecular weight of a protein. These may include post-translational modifications, ...

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Thank you for your enquiry.

I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Aquaporin 4 antibody tested in th...

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I have asked that an FOCR be created for ab9512 of a different lot number. You should be receiving confirmation shortly and it should be included with your next order. Please let me know if you have any questions.

Thank you for contacting us. I would like to your thank customer for their extensive troubleshooting efforts and time. I really do not understand why this particular product has given your customer problem. This product should have worked in their ass...

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