For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to Aquaporin 4
Predicted to work with:
Sheep, Rabbit, Human, Pig, Macaque monkey, Gorilla, Orangutan
Synthetic peptide within Rat Aquaporin 4 aa 250 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH). The exact sequence is proprietary.
Database link: P47863
This antibody gave a positive signal in the following tissue lysates: Rat Brain; Mouse Brain; Rat Cerebellum; Mouse Cerebellum; Mouse Spinal Cord; Rat Spinal Cord; Rat Kidney.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide Constituent: PBS Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 35 kDa).
Forms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
Brain - muscle >> heart, kidney, lung, and trachea.
Belongs to the MIP/aquaporin (TC 1.A.8) family.
Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
Information by UniProt
AQP 4 antibody
Western blot - Anti-Aquaporin 4 antibody (ab156924)
All lanes :
Anti-Aquaporin 4 antibody (ab156924) at 1 µg/ml
Lane 1 :
Brain (Rat) Tissue Lysate
Lane 2 :
Brain (Mouse) Tissue Lysate
Lane 3 :
Rat Cerebellum Neuronal cell lysate
Lane 4 :
Cerebellum Rat Tissue Lysate
Lane 5 :
Spinal Cord (Mouse) Tissue Lysate
Lane 6 :
Spinal Cord (Rat) Tissue Lysate
Lane 7 :
Kidney (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary All lanes :
Goat Anti-Rabbit IgG H&L (HRP) (
) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
48 kDa (
why is the actual band size different from the predicted?
Additional bands at:
125 kDa (possible non-specific binding), 90 kDa (possible non-specific binding)
The predicted molecular weight of Aquaporin 4 is 34 kDa (SwissProt), however we expect to observe a banding pattern at 48 kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab156924 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"