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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Aquaporin 4.
(Peptide available as ab46181.)
Our Abpromise guarantee covers the use of ab46182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 35 kDa).Can be blocked with Human Aquaporin 4 peptide (ab46181). Abcam recommends using milk as the blocking agent.|
|ICC/IF||Use at an assay dependent concentration.|
|IHC-FrFl||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
ab46182 staining Aquaporin 4 in mouse RAW 264.7 cells by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with paraformaldehyde and blocked with 10% serum for 20 minutes at 24°C. Samples were incubated with primary antibody (1/100 in PBS) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit polyclonal (1/1000) was used as the secondary antibody.
Immunohistochemical analysis of Human kidney tissue, labelling Aquaporin 4 with ab46182, diluted 1/250. Shows strong membranous staining of tubular epithelial cells.
Immunocytocchemical analysis of 33B cells originating from Rat nervous tissue oligodendroglioma, labelling AQP4 with ab46182 diluted 1/100. Cells were PFA fixed. Hematoxyling used to counterstained. Negative control given were ab46182 was not included.
Immunohistochemical analysis of Pig brain tissue labelling Aquaporin 4 with ab46182 at 1/100. Tissue fixed by perfussion, antigen retrieval performed by microwaving in citric acid buffer. DAB staining.