Anti-Aquaporin 4 antibody (ab81355)


  • Product nameAnti-Aquaporin 4 antibody
    See all Aquaporin 4 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 4
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    A synthetic peptide corresponding to a sequence at the C-terminal of Human Aquaporin 4

  • Positive control
    • ICC/IF: SKNSH cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products


Our Abpromise guarantee covers the use of ab81355 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 35 kDa.Can be blocked with Aquaporin 4 peptide (ab181722).


  • FunctionForms a water-specific channel. Osmoreceptor which regulates body water balance and mediates water flow within the central nervous system.
  • Tissue specificityBrain - muscle >> heart, kidney, lung, and trachea.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Post-translational
    Phosphorylation by PKC at Ser-180 reduces conductance by 50%. Phosphorylation by PKG at Ser-111 in response to glutamats increases conductance by 40%.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 4 antibody
    • AQP-4 antibody
    • AQP4 antibody
    • AQP4_HUMAN antibody
    • Aquaporin type 4 antibody
    • Aquaporin-4 antibody
    • Aquaporin4 antibody
    • HMIWC 2 antibody
    • HMIWC2 antibody
    • Mercurial insensitive water channel antibody
    • Mercurial-insensitive water channel antibody
    • MGC22454 antibody
    • MIWC antibody
    • WCH 4 antibody
    • WCH4 antibody
    see all

Anti-Aquaporin 4 antibody images

  • ICC/IF image of ab81355 stained SKNSH cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81355, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-Aquaporin 4 antibody (ab81355) at 1 µg/ml

    Lane 1 : Tissue lysates prepared from Rat heart tissue.
    Lane 2 : Tissue lysates prepared from Rat kidney tissue.
    Lane 3 : Tissue lysates prepared from Rat brain tissue.
    Lane 4 : Tissue lysates prepared from Rat brain tissue.

    Lysates/proteins at 50 µg per lane.

    HRP-conjugated Goat anti-rabbit IgG at 1/3000 dilution

    Predicted band size : 35 kDa

References for Anti-Aquaporin 4 antibody (ab81355)

ab81355 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - other (Muscle)
Gel Running Conditions Reduced Denaturing (12)
Loading amount 30 µg
Specification Muscle
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 50% · Temperature: 25°C

Miss. Nathalie Da Silva

Verified customer

Submitted Jul 07 2016

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

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I would like to reassure you that our antibodies have all been tested successfully and would be covered by our guarantee in the applications listed on the datasheets. Therefore, any Aquaporin 4 antibody tested in th...

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