The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2 - 10 µg/ml.
Use a concentration of 0.5 - 1 µg/ml.
Use a concentration of 0.05 - 0.1 µg/ml. Predicted molecular weight: 29 kDa.
Use at an assay dependent concentration. PubMed: 21704616
FunctionForms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
Involvement in diseaseKeratoderma, palmoplantar, Bothnian type
Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
Western blot - Anti-Aquaporin 5 antibody (ab78486)
Lane 1 : Anti-Aquaporin 5 antibody (ab78486) at 0.05 µg/ml Lane 2 : Anti-Aquaporin 5 antibody (ab78486) at 0.1 µg/ml
Lane 1 : rat submaxillary (salivary) gland lysate Lane 2 : rat submaxillary (salivary) gland lysate
Predicted band size : 29 kDa Observed band size : 29 kDa
References for Anti-Aquaporin 5 antibody (ab78486)
This product has been referenced in:
Lin SE et al.
Expression of human carcinoembryonic antigen-related cell adhesion molecule 6 and alveolar progenitor cells in normal and injured lungs of transgenic mice.
Physiol Rep3: (2015).
Read more (PubMed: 26702074) »
Whiteman EL et al. Crumbs3 is essential for proper epithelial development and viability. Mol Cell Biol34:43-56 (2014).
Read more (PubMed: 24164893) »