Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

Overview

  • Product nameAnti-Aquaporin 5 antibody [EPR3747]
    See all Aquaporin 5 primary antibodies
  • Description
    Rabbit monoclonal [EPR3747] to Aquaporin 5
  • Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human Aquaporin 5

  • Positive control
    • Fetal lung and SW480 cell lysates, Human lung tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab92320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.Can be blocked with Aquaporin 5 peptide (ab214008).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these.
ICC/IF 1/100 - 1/250.
  • Application notesIs unsuitable for Flow Cyt or IP.
  • Target

    • FunctionForms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
    • Involvement in diseaseKeratoderma, palmoplantar, Bothnian type
    • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
    • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • Cellular localizationApical cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AQP 5 antibody
      • AQP-5 antibody
      • AQP5 antibody
      • AQP5_HUMAN antibody
      • Aquaporin 5 antibody
      • Aquaporin-5 antibody
      see all

    Anti-Aquaporin 5 antibody [EPR3747] images

    • All lanes : Anti-Aquaporin 5 antibody [EPR3747] (ab92320) at 1/1000 dilution

      Lane 1 : Fetal lung lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 28 kDa
    • ab92320 at 1/100 dilution, staining Aquaporin 5 in paraffin embedded Human lung tissue
    • ab92320 showing positive staining in Normal colon tissue.

    • ab92320 showing positive staining in Normal stomach tissue.

    • ab92320 showing negative staining in Normal brain tissue.

    References for Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

    This product has been referenced in:
    • Jäger J  et al. Human lung tissue explants reveal novel interactions during Legionella pneumophila infections. Infect Immun 82:275-85 (2014). Read more (PubMed: 24166955) »
    • Blaydon DC  et al. Mutations in AQP5, Encoding a Water-Channel Protein, Cause Autosomal-Dominant Diffuse Nonepidermolytic Palmoplantar Keratoderma. Am J Hum Genet N/A:N/A (2013). Read more (PubMed: 23830519) »

    See all 4 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Lung)
    Gel Running Conditions Reduced Denaturing (12% Bis-Tris NuPAGE)
    Loading amount 50 µg
    Specification Lung
    Blocking step Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Pete Serbedzija

    Verified customer

    Submitted Apr 08 2016

    Application Immunocytochemistry/ Immunofluorescence
    Sample Rat Cell (AQP5-EGFP transfected cells / rat lung slices)
    Permeabilization Yes - 0,2 % Saponin
    Specification AQP5-EGFP transfected cells / rat lung slices
    Blocking step Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 01 2015

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step Zytomed Systems HRP Polymer Kit Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step None
    Sample Human Tissue sections (Lung tissue)
    Specification Lung tissue
    Permeabilization No
    Fixative HOPE-Fixation
    Username

    Abcam user community

    Verified customer

    Submitted Mar 12 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (A549)
    Specification A549
    Fixative Paraformaldehyde
    Permeabilization Yes - 0.25% Triton X-100/PBS
    Username

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Thank you for reply.

    I am afraid I will not be able to offer a discount code for the Anti-Prosurfactant Protein B antibody (ab104813) as I am not sure if the antibody is likely to cross react with the chicken protein.

    I can provide ...

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    Thank you for contacting us and sorry for the delay in getting back to you.

    In regards to the Anti-Prosurfactant Protein B antibody (ab104813), I was only able to locate a chicken protein, with the SwissProt reference http://www.uniprot.org/u...

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    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry
    Sample Human Cell (primary alveolar epithelial cells)
    Specification primary alveolar epithelial cells
    Fixative HOPE
    Permeabilization No
    Blocking step Zytomed Systems HRP Kit Blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 31 2012

    Thank you for your enquiry.

    I can confirm that ab92320 antibody is sold as whole antiserum / ascites / tissue culture supernatant. Unpurified antibodies, such as those sold as tissue culture supernatant will not have a concentration stated on ...

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    Thank you for your inquiry.

    I can confirm that this antibody does not react with mouse and rat brain, heart, kidney and spleen samples as tested by our lab in WB.

    I hope this information is helpful and wish you good luck with your e...

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    Thank you for contacting us.

    The cat #92320 is raised against a short peptide, and maps between amino acids 235 and 265 of human Aquaporin 5 (Swiss Prot: P55064).

    I hope this information is helpful to you. Please do not hesitate to co...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"