Recombinant
RabMAb

Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

Overview

  • Product name
    Anti-Aquaporin 5 antibody [EPR3747]
    See all Aquaporin 5 primary antibodies
  • Description
    Rabbit monoclonal [EPR3747] to Aquaporin 5
  • Tested applications
    Suitable for: Flow Cyt, WB, IHC-P, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues within Human Aquaporin 5

  • Positive control
    • Fetal lung and SW480 cell lysates, Human lung tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab92320 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 28 kDa.Can be blocked with Aquaporin 5 peptide (ab214008).
IHC-P 1/100 - 1/250. Antigen retrieval is recommended before commencing with IHC staining protocol. The use of an HRP/AP polymerized secondary antibody is recommended as stronger signals have been found using these.
ICC/IF 1/100 - 1/250.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function
      Forms a water-specific channel. Implicated in the generation of saliva, tears, and pulmonary secretions. Required for TRPV4 activation by hypotonicity (PubMed:16571723). Together with TRPV4, controls regulatory volume decrease in salivary epithelial cells (PubMed:16571723).
    • Involvement in disease
      Keratoderma, palmoplantar, Bothnian type
    • Sequence similarities
      Belongs to the MIP/aquaporin (TC 1.A.8) family.
    • Domain
      Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • Cellular localization
      Apical cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AQP 5 antibody
      • AQP-5 antibody
      • AQP5 antibody
      • AQP5_HUMAN antibody
      • Aquaporin 5 antibody
      • Aquaporin-5 antibody
      see all

    Anti-Aquaporin 5 antibody [EPR3747] images

    • Flow cytometry analysis of SW480 human colorectal adenocarcinoma ) cells labeling with unpurified ab92320 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

    • ab92320 showing positive staining in Normal colon tissue.

    • All lanes : Anti-Aquaporin 5 antibody [EPR3747] (ab92320) at 1/1000 dilution

      Lane 1 : Fetal lung lysate
      Lane 2 : SW480 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit IgG at 1/1000 dilution

      Predicted band size : 28 kDa
    • ab92320 at 1/100 dilution, staining Aquaporin 5 in paraffin embedded Human lung tissue
    • ab92320 showing positive staining in Normal stomach tissue.

    • ab92320 showing negative staining in Normal brain tissue.

    References for Anti-Aquaporin 5 antibody [EPR3747] (ab92320)

    This product has been referenced in:
    • Liebler JM  et al. Combinations of differentiation markers distinguish subpopulations of alveolar epithelial cells in adult lung. Am J Physiol Lung Cell Mol Physiol 310:L114-20 (2016). IHC-P . Read more (PubMed: 26545903) »
    • Jäger J  et al. Human lung tissue explants reveal novel interactions during Legionella pneumophila infections. Infect Immun 82:275-85 (2014). Read more (PubMed: 24166955) »

    See all 5 Publications for this product

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (intervertebral disc)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    intervertebral disc
    Blocking step
    1% BSA in 75% TBS 25% serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 25% · Temperature: 18°C
    Fixative
    10% neutral buffered formalin
    Username

    Abcam user community

    Verified customer

    Submitted Jun 05 2017

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (Lung)
    Gel Running Conditions
    Reduced Denaturing (12% Bis-Tris NuPAGE)
    Loading amount
    50 µg
    Specification
    Lung
    Blocking step
    Odyssey TBS Blocking Buffer (proprietary) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 23°C
    Username

    Pete Serbedzija

    Verified customer

    Submitted Apr 08 2016

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Rat Cell (AQP5-EGFP transfected cells / rat lung slices)
    Permeabilization
    Yes - 0,2 % Saponin
    Specification
    AQP5-EGFP transfected cells / rat lung slices
    Blocking step
    Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Oct 01 2015

    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Zytomed Systems HRP Polymer Kit Blocking Solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Antigen retrieval step
    None
    Sample
    Human Tissue sections (Lung tissue)
    Specification
    Lung tissue
    Permeabilization
    No
    Fixative
    HOPE-Fixation
    Username

    Abcam user community

    Verified customer

    Submitted Mar 12 2015

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (A549)
    Specification
    A549
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.25% Triton X-100/PBS
    Username

    Abcam user community

    Verified customer

    Submitted Apr 05 2013

    Thank you for reply.

    I am afraid I will not be able to offer a discount code for the Anti-Prosurfactant Protein B antibody (ab104813) as I am not sure if the antibody is likely to cross react with the chicken protein.

    I can provide ...

    Read More

    Thank you for contacting us and sorry for the delay in getting back to you.

    In regards to the Anti-Prosurfactant Protein B antibody (ab104813), I was only able to locate a chicken protein, with the SwissProt reference http://www.uniprot.org/u...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Human Cell (primary alveolar epithelial cells)
    Specification
    primary alveolar epithelial cells
    Fixative
    HOPE
    Permeabilization
    No
    Blocking step
    Zytomed Systems HRP Kit Blocking solution as blocking agent for 5 minute(s) · Concentration: 100% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Jul 31 2012

    Thank you for your enquiry.

    I can confirm that ab92320 antibody is sold as whole antiserum / ascites / tissue culture supernatant. Unpurified antibodies, such as those sold as tissue culture supernatant will not have a concentration stated on ...

    Read More

    Thank you for your inquiry.

    I can confirm that this antibody does not react with mouse and rat brain, heart, kidney and spleen samples as tested by our lab in WB.

    I hope this information is helpful and wish you good luck with your e...

    Read More

    1-10 of 13 Abreviews or Q&A

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