Anti-Aquaporin 8 antibody (ab15124)

Overview

  • Product nameAnti-Aquaporin 8 antibody
    See all Aquaporin 8 primary antibodies
  • Description
    Rabbit polyclonal to Aquaporin 8
  • SpecificityThe 16 AA peptide was found unique to AQP8 without significant homology to any other AQPs
  • Tested applicationsSuitable for: WB, ELISAmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    A 16 aa synthetic peptide (Rat) (C terminal). The domain is predicted to be cytoplasmic.

Properties

Applications

Our Abpromise guarantee covers the use of ab15124 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/10000 - 1/50000.
    WB: 1/1000 - 1/5000. Predicted molecular weight: 29 kDa. Use of 0.5-1% milk in all primary/secondary antibody-enzyme conjugate incubations is recommended in order to suppress non-specific bands.

    Is unsuitable for IHC.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionForms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.
    • Tissue specificityExpressed only in pancreas and colon.
    • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
    • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
    • Cellular localizationMembrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • AQP 8 antibody
      • AQP-8 antibody
      • AQP8 antibody
      • AQP8_HUMAN antibody
      • Aquaporin 8 antibody
      • Aquaporin-8 antibody
      • Aquaporin8 antibody
      see all

    References for Anti-Aquaporin 8 antibody (ab15124)

    ab15124 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application Western blot
    Sample Pig Tissue lysate - whole (colon)
    Loading amount 60 µg
    Specification colon
    Gel Running Conditions Reduced Denaturing (12% gel)
    Blocking step Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

    Abcam user community

    Verified customer

    Submitted Oct 21 2011

    Thank you for your enquiry and I'm sorry to hear that you are experiencing difficulty with ab15124. Are you using human samples? What dilutions of ab15124 have you tried? At this point I would suggest using it at a 1:1000 dilution and incubate overnigh...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"