Overview

  • Product nameAnti-Aquaporin 8 antibody
    See all Aquaporin 8 primary antibodies
  • Description
    Mouse monoclonal to Aquaporin 8
  • Tested applicationsSuitable for: WB, IHC-P, Flow Cyt, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length human Aquaporin 8 protein (AAH40630) with a 26 kDa tag.

  • Positive control
    • IMR-32 cell lysate; human colon tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab77198 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 27 kDa.
IHC-P Use a concentration of 3 µg/ml.
Flow Cyt Use 1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ELISA Use at an assay dependent concentration. Detection limit for recombinant tagged Aquaporin 8 is approximately 10ng/ml if used as a capture antibody.

Target

  • FunctionForms a water-specific channel; mercury-sensitive. Not permeable to glycerol or urea.
  • Tissue specificityExpressed only in pancreas and colon.
  • Sequence similaritiesBelongs to the MIP/aquaporin (TC 1.A.8) family.
  • DomainAquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AQP 8 antibody
    • AQP-8 antibody
    • AQP8 antibody
    • AQP8_HUMAN antibody
    • Aquaporin 8 antibody
    • Aquaporin-8 antibody
    • Aquaporin8 antibody
    see all

Anti-Aquaporin 8 antibody images

  • Anti-Aquaporin 8 antibody (ab77198) at 5 µg/ml + IMR-32 cell lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 27 kDa
    Observed band size : 27 kDa
  • Immunoperoxidase analysis of Aquaporin 8 in formalin-fixed, paraffin-embedded human colon tissue using 3 µg/ml of ab77198.
  • Overlay histogram showing HCT116 cells stained with ab77198 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77198, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

References for Anti-Aquaporin 8 antibody (ab77198)

ab77198 has not yet been referenced specifically in any publications.

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