Overview

  • Product name
  • Description
    Rabbit polyclonal to ARAP1
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Cow, Dog, Pig, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
  • Immunogen

    Synthetic peptide, corresponding to a region between amino acids 1160-1210 of Human ARAP1 (NP_056057.1).

  • Positive control
    • HeLa whole cell lysate.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab99382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 136 kDa.
IP Use at 2-5 µg/mg of lysate.

Target

  • Function
    Phosphatidylinositol-3,4,5-trisphosphate-dependent GTPase-activating protein that modulates actin cytoskeleton remodeling by regulating ARF and RHO family members. Is activated by phosphatidylinositol-3,4,5-trisphosphate (PtdIns(3,4,5)P3) binding. Can be activated by phosphatidylinositol-3,4-bisphosphate (PtdIns(3,4,5)P2) binding, albeit with lower efficiency. Has a preference for ARF1 and ARF5.
  • Tissue specificity
    Detected in heart, skeletal muscle, spleen, kidney, liver, placenta, lung, peripheral blood leukocytes, adrenal gland, bone marrow, brain, lymph node, mammary gland, prostate, spinal cord, stomach, thyroid and trachea.
  • Sequence similarities
    Contains 1 Arf-GAP domain.
    Contains 4 PH domains.
    Contains 1 Ras-associating domain.
    Contains 1 Rho-GAP domain.
    Contains 1 SAM (sterile alpha motif) domain.
  • Cellular localization
    Cytoplasm. Golgi apparatus > Golgi stack membrane. Cell membrane. Associated with Golgi stacks in resting cells. Throughout the cytoplasm and in surface protrusion in cells that are in the process of attaching to a surface and spreading.
  • Information by UniProt
  • Database links
  • Alternative names
    • 2410002L19Rik antibody
    • ANK repeat and PH domain-containing protein 1 antibody
    • ARAP 1 antibody
    • Arap1 antibody
    • ARAP1_HUMAN antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domains containing protein 1 antibody
    • Arf GAP Rho GAP ankyrin repeat and pleckstrin homology domain containing protein 1 antibody
    • Arf-GAP with Rho-GAP domain antibody
    • CENT D2 antibody
    • Centaurin delta 2 antibody
    • Centaurin delta2 antibody
    • Centaurin-delta-2 antibody
    • CENTD 2 antibody
    • CENTD2 antibody
    • Cnt d2 antibody
    • Cnt-d2 antibody
    • Cntd2 antibody
    • KIAA0782 antibody
    • MGC176140 antibody
    • mKIAA0782 antibody
    see all

Images

  • All lanes : Anti-ARAP1 antibody (ab99382) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 136 kDa


    Exposure time : 30 seconds
  • Detection of ARAP1 by Western Blot of Immunprecipitate.
    ab99382 at 1µg/ml staining ARAP1 in HeLa whole cell lysate immunoprecipitated using ab99382 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 10 seconds.

References

ab99382 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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