Overview

  • Product nameAnti-ARAP3 antibody
  • Description
    Goat polyclonal to ARAP3
  • Tested applicationsSuitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide:

    CTSSPPSSQPLT

    , corresponding to C terminal amino acids 1533-1544 of Human ARAP3.

  • General notesGenBank Accession Number – NP_071926

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab5911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution. PubMed: 21076469
IP Use at an assay dependent dilution. PubMed: 21076469
WB Use at an assay dependent dilution. Can be blocked with Human ARAP3 peptide (ab23118). PubMed: 21076469

Target

  • RelevanceThe ARAP3 gene encodes a phosphoinositide binding protein containing ARF-GAP, RHO-GAP, RAS-associating, and pleckstrin homology domains. The ARF-GAP and RHO-GAP domains cooperate in mediating rearrangements in the cell cytoskeleton and cell shape.
  • Database links
  • Alternative names
    • Ankyrin repeat and plekstrin homology domains containing protein 3 antibody
    • ARAP 3 antibody
    • ARF GAP antibody
    • ARF-GAP RHO-GAP ankyrin repeat and plekstrin homology domains containing protein 3 antibody
    • CENTD3 antibody
    • FLJ21065 antibody
    • Phosphoinositide binding protein antibody
    • PtdIns 3 4 5 P3 binding protein antibody
    • RHO GAP antibody
    see all

Anti-ARAP3 antibody images

  • All lanes : Anti-ARAP3 antibody (ab5911) at 1/500 dilution

    Lane 1 : HEK293 negative control
    Lane 2 : HEK293 overexpressed GFP-ARAP3
    Lane 3 : Whole tissue lysate prepared from mouse heart
    Lane 4 : Whole tissue lysate prepared from mouse brain
    Lane 5 : Whole tissue lysate prepared from mouse diaphragma
    Lane 6 : Whole tissue lysate prepared from mouse fundus
    Lane 7 : Whole tissue lysate prepared from rabbit heart

    Lysates/proteins at 100 µg per lane.

    Secondary
    Alexa-Fluor 680 conjugated donkey polyclonal at 1/10000 dilution

    Image courtesy of an anonymous Abreview.

    See Abreview

References for Anti-ARAP3 antibody (ab5911)

This product has been referenced in:
  • Yagi R  et al. ARAP3 inhibits peritoneal dissemination of scirrhous gastric carcinoma cells by regulating cell adhesion and invasion. Oncogene : (2010). WB, IHC-P, IP ; Human . Read more (PubMed: 21076469) »

See 1 Publication for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (HEART. BRAIN,DIAPHRAGMA,FUNDUS)
Loading amount 100 µg
Specification HEART. BRAIN,DIAPHRAGMA,FUNDUS
Gel Running Conditions Reduced Denaturing (7.5%)
Blocking step Oddysey blocking buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Username

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Verified customer

Submitted May 02 2012

Thank you for your call today and for your questions. I will let you know once I have more information about the homology of the ab67278 immunogen to the murine protein. Please see below for the discount codes to test ab121972 and ab5911 in mouse, and ...

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Thank you for your enquiry. Thus far, we have not received any feedback concerning this antibody. All the information that we currently have is on the online datasheet. Ab5911 is a Fast Track antibody: Fast-Track antibodies are immunogen affinity pu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"