Anti-ARD1A antibody (ab155687)
Key features and details
- Rabbit polyclonal to ARD1A
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-ARD1A antibody
See all ARD1A primary antibodies -
Description
Rabbit polyclonal to ARD1A -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-235 of Human ARD1A (UniProt: P41227).
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Positive control
- H1299 cell lysates, transfected with scramble siRNA and ARDIA siRNA; NIH 3T3, JC, BCL1, C2C12 and RAW264.7 whole cell lysates; HeLa cells; Human PC13 xenograft tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155687 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
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IHC-P |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 26 kDa. |
IHC-P
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the acetyltransferase family. ARD1 subfamily.
Contains 1 N-acetyltransferase domain. -
Post-translational
modificationsCleaved by caspases during apoptosis. -
Cellular localization
Cytoplasm. Nucleus. According to PubMed:12464182 it is cytoplasmic. According to PubMed:15496142, it is nuclear and cytoplasmic. Also present in the free cytosolic and cytoskeleton-bound polysomes. - Information by UniProt
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Database links
- Entrez Gene: 8260 Human
- Entrez Gene: 56292 Mouse
- Omim: 300013 Human
- SwissProt: P41227 Human
- SwissProt: Q9QY36 Mouse
- Unigene: 433291 Human
- Unigene: 246654 Mouse
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Alternative names
- Alpha N acetyltransferase 1A antibody
- ARD1 antibody
- ARD1 homolog N acetyltransferase (S. cerevisiae) antibody
see all
Images
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All lanes : Anti-ARD1A antibody (ab155687) at 1/5000 dilution
Lane 1 : H1299 cell lysate, transfected with scramble siRNA
Lane 2 : H1299 cell lysate, transfected with ARDIA siRNA
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
All lanes : Anti-ARD1A antibody (ab155687) at 1/1000 dilution
Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lane 4 : C2C12 whole cell lysate
Lane 5 : RAW264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 26 kDa
12% SDS PAGE -
Immunofluorescent analysis of methanol-fixed HeLa cells (100% for 5 mins) labeling ARD1A with ab155687 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
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Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue labeling ARD1A with ab155687 at 1/500 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab155687 has been referenced in 2 publications.
- Kweon HY et al. Naa12 compensates for Naa10 in mice in the amino-terminal acetylation pathway. Elife 10:N/A (2021). PubMed: 34355692
- Yang H et al. microRNA-342-5p and miR-608 inhibit colon cancer tumorigenesis by targeting NAA10. Oncotarget 7:2709-20 (2016). PubMed: 26646451