Overview

  • Product name
  • Description
    Rabbit polyclonal to ARD1A
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-235 of Human ARD1A (UniProt: P41227).

  • Positive control
    • H1299 cell lysates, transfected with scramble siRNA and ARDIA siRNA; NIH 3T3, JC, BCL1, C2C12 and RAW264.7 whole cell lysates; HeLa cells; Human PC13 xenograft tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab155687 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 26 kDa.
IHC-P 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function
    In complex with NAA15, displays alpha (N-terminal) acetyltransferase activity. Without NAA15, displays epsilon (internal) acetyltransferase activity towards HIF1A, thereby promoting its degradation.
  • Tissue specificity
    Ubiquitous.
  • Sequence similarities
    Belongs to the acetyltransferase family. ARD1 subfamily.
    Contains 1 N-acetyltransferase domain.
  • Post-translational
    modifications
    Cleaved by caspases during apoptosis.
  • Cellular localization
    Cytoplasm. Nucleus. According to PubMed:12464182 it is cytoplasmic. According to PubMed:15496142, it is nuclear and cytoplasmic. Also present in the free cytosolic and cytoskeleton-bound polysomes.
  • Information by UniProt
  • Database links
  • Alternative names
    • Alpha N acetyltransferase 1A antibody
    • ARD1 antibody
    • ARD1 homolog N acetyltransferase (S. cerevisiae) antibody
    • ARD1 homolog A N acetyltransferase (S. cerevisiae) antibody
    • ARD1 homolog A N acetyltransferase antibody
    • ARD1A antibody
    • DXS707 antibody
    • MGC71248 antibody
    • N acetyltransferase ARD1, human homolog of antibody
    • N alpha acetyltransferase 10 NatA catalytic subunit antibody
    • N terminal acetyltransferase complex ARD1 subunit homolog A antibody
    • N(alpha) acetyltransferase 10 NatA catalytic subunit antibody
    • N-alpha-acetyltransferase 10 antibody
    • N-terminal acetyltransferase complex ARD1 subunit homolog A antibody
    • Naa10 antibody
    • NAA10_HUMAN antibody
    • NatA catalytic subunit antibody
    • TE2 antibody
    see all

Images

  • All lanes : Anti-ARD1A antibody (ab155687) at 1/5000 dilution

    Lane 1 : H1299 cell lysate, transfected with scramble siRNA
    Lane 2 : H1299 cell lysate, transfected with ARDIA siRNA

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • All lanes : Anti-ARD1A antibody (ab155687) at 1/1000 dilution

    Lane 1 : NIH 3T3 whole cell lysate
    Lane 2 : JC whole cell lysate
    Lane 3 : BCL1 whole cell lysate
    Lane 4 : C2C12 whole cell lysate
    Lane 5 : RAW264.7 whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 26 kDa



    12% SDS PAGE
  • Immunofluorescent analysis of methanol-fixed HeLa cells (100% for 5 mins) labeling ARD1A with ab155687 at 1/500 dilution. Lower image shows cells co-stained with Hoechst 33342.
  • Immunohistochemical analysis of paraffin-embedded Human PC13 xenograft tissue labeling ARD1A with ab155687 at 1/500 dilution.

References

ab155687 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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