Anti-ZEB1 antibody [OTI3G6] - N-terminal (ab180905)
Key features and details
- Mouse monoclonal [OTI3G6] to ZEB1 - N-terminal
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-ZEB1 antibody [OTI3G6] - N-terminal
See all ZEB1 primary antibodies -
Description
Mouse monoclonal [OTI3G6] to ZEB1 - N-terminal -
Host species
Mouse -
Specificity
Does not cross react with ZEB2. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken -
Immunogen
Recombinant fragment corresponding to Human ZEB1 aa 1-350 (N terminal). Produced in E.coli (NP_110378).
Database link: P37275 -
Positive control
- IHC-P: Human lung carcinoma, breast adenocarcinoma, kidney carcinoma, endometrium adenocarcinoma, prostate and lymph node tissues.
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General notes
Dilute in PBS (pH7.3) if necessary.
The clone number has been updated from 3G6 to OTI3G6, both clone numbers name the same clone.
This product was changed from ascites to tissue culture supernatant on 6th September 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA, 48% PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified from TCS -
Clonality
Monoclonal -
Clone number
OTI3G6 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab180905 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB | (1) |
Use at an assay dependent concentration.
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IHC-P | (1) |
1/150.
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Notes |
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WB
Use at an assay dependent concentration. |
IHC-P
1/150. |
Target
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Function
Inhibits interleukin-2 (IL-2) gene expression. May be responsible for transcriptional repression of the IL-2 gene. Enhances or represses the promoter activity of the ATP1A1 gene depending on the quantity of cDNA and on the cell type. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Promotes tumorigenicity by repressing stemness-inhibiting microRNAs. -
Tissue specificity
Colocalizes with SMARCA4/BRG1 in E-cadherin-negative cells from established lines, and stroma of normal colon as well as in de-differentiated epithelial cells at the invasion front of colorectal carcinomas (at protein level). Expressed in heart and skeletal muscle, but not in liver, spleen, or pancreas. -
Involvement in disease
Defects in ZEB1 are the cause of posterior polymorphous corneal dystrophy type 3 (PPCD3) [MIM:609141]. PPCD is a rare disease involving metaplasia and overgrowth of corneal endothelial cells. In patients with PPCD, these cells manifest in an epithelial morphology and gene expression pattern, produce an aberrant basement membrane, and, sometimes, spread over the iris and nearby structures in a way that increases the risk for glaucoma.
Defects in ZEB1 are the cause of corneal dystrophy Fuchs endothelial type 6 (FECD6) [MIM:613270]. It is an ocular disorder caused by loss of endothelium of the central cornea. It is characterized by focal wart-like guttata that arise from Descemet membrane and develop in the central cornea, epithelial blisters, reduced vision and pain. Descemet membrane is thickened by abnormal collagenous deposition. -
Sequence similarities
Belongs to the delta-EF1/ZFH-1 C2H2-type zinc-finger family.
Contains 7 C2H2-type zinc fingers.
Contains 1 homeobox DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6935 Human
- Entrez Gene: 21417 Mouse
- Entrez Gene: 25705 Rat
- Omim: 189909 Human
- SwissProt: P37275 Human
- SwissProt: Q64318 Mouse
- SwissProt: Q62947 Rat
- Unigene: 124503 Human
see all -
Alternative names
- AREB 6 antibody
- AREB6 antibody
- BZP antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue labeling ZEB1 with ab180905. Heat mediated antigen retrieval was performed using 10mM citrate buffer pH 6 for 3 minutes at 120°C.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast adenocarcinoma tissue labeling ZEB1 with ab180905. Heat mediated antigen retrieval was performed using 10mM citrate buffer pH 6 for 3 minutes at 120°C.
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Immunohistochemical analysis of paraffin-embedded Human lymph node tissue labeling ZEB1 with ab180905 at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling ZEB1 with ab180905 at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human endometrium adenocarcinoma tissue labeling ZEB1 with ab180905 at 1/150 dilution.
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Immunohistochemical analysis of paraffin-embedded Human lung carcinoma tissue labeling ZEB1 with ab180905 at 1/150 dilution.
Datasheets and documents
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SDS download
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Datasheet download
References (19)
ab180905 has been referenced in 19 publications.
- Li J et al. METTL3 promotes prostatic hyperplasia by regulating PTEN expression in an m6A-YTHDF2-dependent manner. Cell Death Dis 13:723 (2022). WB ; Human . PubMed: 35985997
- Tanimura K et al. HER3 activation contributes toward the emergence of ALK inhibitor-tolerant cells in ALK-rearranged lung cancer with mesenchymal features. NPJ Precis Oncol 6:5 (2022). PubMed: 35042943
- Gött H et al. ZEB1 induces N-cadherin expression in human glioblastoma and may alter patient survival. Mol Clin Oncol 17:123 (2022). PubMed: 35911664
- Khadri FZ et al. Impact of Epithelial-Mesenchymal Transition on the Immune Landscape in Breast Cancer. Cancers (Basel) 13:N/A (2021). PubMed: 34680248
- Wang F et al. ZEB1 promotes colorectal cancer cell invasion and disease progression by enhanced LOXL2 transcription. Int J Clin Exp Pathol 14:9-23 (2021). PubMed: 33532019