The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100. Predicted molecular weight: 21 kDa.
ADP ribosylation factors (ARFs)1 are small G proteins of the Ras superfamily involved in intracellular trafficking. ARF1 functions in the formation of transport vesicles. ARF1 controls the binding to Golgi membranes of coatomer, a large (700 kDa) cytoplasmic complex, which upon oligomerization deforms the lipid bilayer and induces the formation of "COPI" coated vesicles. In addition, ARF1 activates phospholipase D. ARF1 may recruit coatomer to the membrane based on the ability of ARF1 to interact directly, simultaneously, and in a GTP dependent manner both with its protein target and with lipid membranes. This dual interaction can be ascribed to two regions of ARF1. The classical switch I and II regions of the "Raslike" core domain of ARF1 are probably the main determinants of its interaction with coatomer, whereas the interaction of ARF1 with membrane lipids involves a region specific to ARF1: the N terminal helix, which is amphipathic and myristoylated. Three human ARF
genes have been isolated that share high homology: 96% between ARF1 and 3; 84% between both ARF3 and 4 and ARF 1 and 4. There is consistency between ARFs between species.