Overview

  • Product name
    Anti-ARF 1+3 antibody
  • Description
    Rabbit polyclonal to ARF 1+3
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    KLH-conjugated synthetic peptide selected from the Center region of Human ARF 1+3.

  • Positive control
    • MCF-7 cell line lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab91169 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Predicted molecular weight: 21 kDa.
ELISA 1/1000.

Target

  • Relevance
    ADP ribosylation factors (ARFs)1 are small G proteins of the Ras superfamily involved in intracellular trafficking. ARF1 functions in the formation of transport vesicles. ARF1 controls the binding to Golgi membranes of coatomer, a large (700 kDa) cytoplasmic complex, which upon oligomerization deforms the lipid bilayer and induces the formation of "COPI" coated vesicles. In addition, ARF1 activates phospholipase D. ARF1 may recruit coatomer to the membrane based on the ability of ARF1 to interact directly, simultaneously, and in a GTP dependent manner both with its protein target and with lipid membranes. This dual interaction can be ascribed to two regions of ARF1. The classical switch I and II regions of the "Raslike" core domain of ARF1 are probably the main determinants of its interaction with coatomer, whereas the interaction of ARF1 with membrane lipids involves a region specific to ARF1: the N terminal helix, which is amphipathic and myristoylated. Three human ARF genes have been isolated that share high homology: 96% between ARF1 and 3; 84% between both ARF3 and 4 and ARF 1 and 4. There is consistency between ARFs between species.
  • Cellular localization
    Golgi Apparatus
  • Database links
  • Alternative names
    • ADP ribosylation factor 1 antibody
    • ADP ribosylation factor 3 antibody
    • ARF1 antibody
    • ARF3 antibody

Anti-ARF 1+3 antibody images

  • Anti-ARF 1+3 antibody (ab91169) at 1/50 dilution + MCF-7 cell line lysate at 35 µg

    Predicted band size : 21 kDa

References for Anti-ARF 1+3 antibody (ab91169)

ab91169 has not yet been referenced specifically in any publications.

Product Wall

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (kidney, epithelial cells)
Loading amount
100000 cells
Specification
kidney, epithelial cells
Treatment
transfected (or not) with ARF3-HA and infected (or not) with influenza A virus
Gel Running Conditions
Reduced Denaturing (15%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

Submitted Apr 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (kidney, epithelial cells)
Specification
kidney, epithelial cells
Fixative
Formaldehyde
Permeabilization
Yes - 0.2% triton x100 in PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

Submitted Apr 03 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (Panc-1 cell)
Loading amount
60 µg
Specification
Panc-1 cell
Gel Running Conditions
Reduced Denaturing (8% SDS-PAGE)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Sep 09 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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