The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally.
WB, IHC-P, ICC/IFmore details Unsuitable for:
Flow Cyt or IP
Mouse, Rat, Human
Synthetic peptide within Human ARF1 aa 1-100. The exact sequence is proprietary.
Human fetal kidney, Human fetal brain and MCF7 lysates; Human breast ductal carcinoma, and Human colonic adenocarcinoma tissues
This antibody gave a positive result when used in the following methanol fixed cell lines: CaCo2
This product is a recombinant rabbit monoclonal antibody.
ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Western blot - Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)
All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate Lane 2 : Human fetal brain lysate Lane 3 : MCF7 lysate
Rafiq NB et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol216:181-197 (2017).
Read more (PubMed: 28007915) »
Eiseler T et al. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem291:462-77 (2016).
Read more (PubMed: 26507660) »