Recombinant
RabMAb

Anti-ARF1 + ARF3 antibody [EPR443] (ab108347)

Overview

  • Product name
    Anti-ARF1 + ARF3 antibody [EPR443]
  • Description
    Rabbit monoclonal [EPR443] to ARF1 + ARF3
  • Specificity
    The immunogen used to raise this antibody shares 100% homology with human ARF3, 96% homology with ARF4 and ARF5, and 88% homology with ARF6. Cross-reactivity with the latter three proteins have not been confirmed experimentally.
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARF1 aa 1-100. The exact sequence is proprietary.

  • Positive control
    • Human fetal kidney, Human fetal brain and MCF7 lysates; Human breast ductal carcinoma, and Human colonic adenocarcinoma tissues This antibody gave a positive result when used in the following methanol fixed cell lines: CaCo2
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Alternative versions available:

    Anti-ARF1 antibody (Alexa Fluor® 647) [EPR443] (ab207645)

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

Properties

Applications

Our Abpromise guarantee covers the use of ab108347 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/50 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    Images

    • All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/50000 dilution

      Lane 1 : Full length human ARF-1 recombinant protein
      Lane 2 : Full length human ARF-3 recombinant protein

      Lysates/proteins at 0.015 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 21 kDa
      Observed band size : 23 kDa (why is the actual band size different from the predicted?)


      Exposure time : 3 minutes

      Blocking and diluting buffer: 5% NFDM /TBST

      Human ARF-1 recombinant protein, (Cat#:ab123538 )
      Human ARF-3 recombinant protein, (Cat#:ab113581 )

    • ICC/IF image of ab108347 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab108347 at 1/100 dilution overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • All lanes : Anti-ARF1 + ARF3 antibody [EPR443] (ab108347) at 1/1000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal brain lysate
      Lane 3 : MCF7 lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 21 kDa
    • Immunohistochemical analysis of ARF1 in paraffin embedded Human breast ductal carcinoma tissue, using ab108347 at a dilution of 1/100.
    • Immunohistochemical analysis of ARF1 in paraffin embedded Human colonic adenocarcinoma tissue, using ab108347 at a dilution of 1/100.

    References

    This product has been referenced in:
    • Rafiq NB  et al. Podosome assembly is controlled by the GTPase ARF1 and its nucleotide exchange factor ARNO. J Cell Biol 216:181-197 (2017). WB . Read more (PubMed: 28007915) »
    • Eiseler T  et al. Protein Kinase D2 Assembles a Multiprotein Complex at the Trans-Golgi Network to Regulate Matrix Metalloproteinase Secretion. J Biol Chem 291:462-77 (2016). Read more (PubMed: 26507660) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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