Overview

  • Product name
  • Description
    Rabbit polyclonal to ARF1
  • Tested applications
    Suitable for: ICC/IF, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Dog, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Synthetic peptide corresponding to Human ARF1 aa 171-181 (C terminal).
    Sequence:

    DWLSNQLRNQK


    Database link: P84077

  • Positive control
    • HeLa, U2 OS, 3T3, NRK and MDCK cell lysates; HeLa cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab183576 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100 - 1/200.
IP Use at 4 µg/mg of lysate.
WB 1/1000 - 1/2000. Predicted molecular weight: 21 kDa.

Target

Images

  • All lanes : Anti-ARF1 antibody (ab183576) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : U2 OS cell lysate
    Lane 3 : 3T3 cell lysate
    Lane 4 : NRK cell lysate
    Lane 5 : MDCK cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    goat anti-rabbit IgG-HRP at 1/1500 dilution
    Developed using the ECL technique

    Predicted band size : 21 kDa
  • Detection of ARF1 in Immunoprecipitates of HeLa cell lysate. Antigen-antibody complexes were formed by incubating 500μg of HeLa whole cell lysate with 2μg ab183576 (lane 2) compared with HeLa cell lysate as a positive control (lane 1). For detection, ab183576 was used at 1/1000 dilution.

  • Immunofluorescent analysis of HeLa cells (formalin-fixed, 0.1% Triton X-100 permeabilized) labeling ARF1 with ab183576 at 1/100 dilution followed with DyLight 488 goat anti-rabbit IgG secondary antibody at 1/400 dilution. Nuclei (blue) were stained with Hoechst 33342 dye.

References

ab183576 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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