Overview

  • Product name
  • Description
    Goat polyclonal to ARF1
  • Tested applications
    Suitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
    Predicted to work with: Xenopus laevis, Cynomolgus monkey
  • Immunogen

    Synthetic peptide:

    EGLDWLSNQLRNQK

    by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1

  • Positive control
    • HEK293, A431 and HepG2 cells

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notes
    This antibody is expected to recognize all four reported isoforms.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab58578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
ELISA 1/8000.

Target

Images

References

This product has been referenced in:
  • Humphreys D  et al. Inhibition of WAVE Regulatory Complex Activation by a Bacterial Virulence Effector Counteracts Pathogen Phagocytosis. Cell Rep 17:697-707 (2016). Read more (PubMed: 27732847) »
  • Humphreys D  et al. Arf6 coordinates actin assembly through the WAVE complex, a mechanism usurped by Salmonella to invade host cells. Proc Natl Acad Sci U S A 110:16880-5 (2013). Read more (PubMed: 24085844) »

See all 5 Publications for this product

Customer reviews and Q&As

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Dog Cell lysate - whole cell (MDCK)
Loading amount
15 µg
Specification
MDCK
Gel Running Conditions
Reduced Denaturing (15% tris-glycine)
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Cow Cell lysate - whole cell (Bovine kidney cells)
Loading amount
15 µg
Specification
Bovine kidney cells
Gel Running Conditions
Reduced Denaturing (15 % SDS-PAGE)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Recombinant protein (Human recombinant Arf-1 expressed in E.coli)
Loading amount
10 µg
Specification
Human recombinant Arf-1 expressed in E.coli
Gel Running Conditions
Reduced Denaturing (10% Tris-glycine)
Blocking step
Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2012

Application
Western blot
Sample
Xenopus laevis Cell lysate - other (Xenopus laevis egg extract)
Loading amount
5 µg
Specification
Xenopus laevis egg extract
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Anne-Lore Schlaitz

Verified customer

Submitted Jan 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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