Overview

  • Product nameAnti-ARF1 antibody
    See all ARF1 primary antibodies
  • Description
    Goat polyclonal to ARF1
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Dog, Human
    Predicted to work with: Xenopus laevis, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    EGLDWLSNQLRNQK

    by a Cysteine residue linker, corresponding to C terminal amino acids 168-181 of ARF1

  • Positive control
    • HEK293, A431 and HepG2 cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Primary antibody notesThis antibody is expected to recognize all four reported isoforms.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab58578 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 21 kDa).
ELISA 1/8000.

Target

Anti-ARF1 antibody images

References for Anti-ARF1 antibody (ab58578)

This product has been referenced in:
  • Koronakis V  et al. WAVE regulatory complex activation by cooperating GTPases Arf and Rac1. Proc Natl Acad Sci U S A 108:14449-54 (2011). WB . Read more (PubMed: 21844371) »
  • Sharma P  et al. Endoplasmic reticulum protein targeting of phospholamban: a common role for an N-terminal di-arginine motif in ER retention? PLoS One 5:e11496 (2010). WB ; Human . Read more (PubMed: 20634894) »

See all 3 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Dog Cell lysate - whole cell (MDCK)
Loading amount 15 µg
Specification MDCK
Gel Running Conditions Reduced Denaturing (15% tris-glycine)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Abcam user community

Verified customer

Submitted Nov 19 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Cell lysate - whole cell (Bovine kidney cells)
Loading amount 15 µg
Specification Bovine kidney cells
Gel Running Conditions Reduced Denaturing (15 % SDS-PAGE)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

Submitted Nov 06 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Recombinant protein (Human recombinant Arf-1 expressed in E.coli)
Loading amount 10 µg
Specification Human recombinant Arf-1 expressed in E.coli
Gel Running Conditions Reduced Denaturing (10% Tris-glycine)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 30 2012

Application Western blot
Sample Xenopus laevis Cell lysate - other (Xenopus laevis egg extract)
Loading amount 5 µg
Specification Xenopus laevis egg extract
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. Anne-Lore Schlaitz

Verified customer

Submitted Jan 28 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"