Overview

  • Product name
  • Description
    Rabbit polyclonal to ARF1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Pig, Zebrafish
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-142 of Human ARF1 (AAH09247).

  • Positive control
    • A431 and H1299 whole cell lysates; 293T, HeLa, HepG2, MOLT4 and Raji cell lysates

Properties

Applications

Our Abpromise guarantee covers the use of ab96791 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.

Target

  • Function
    GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Arf family.
  • Cellular localization
    Golgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP Ribosylation Factor 1 antibody
    • ADP-ribosylation factor 1 antibody
    • ARF 1 antibody
    • ARF1 antibody
    • ARF1_HUMAN antibody
    see all

Images

  • All lanes : Anti-ARF1 antibody (ab96791) at 1/1000 dilution

    Lane 1 : A431 whole cell lysate
    Lane 2 : H1299 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 21 kDa

References

ab96791 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab96791.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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