Anti-ARF1 antibody [ARFS 5F2/2] (ab18109)

Overview

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab18109 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.

Target

  • FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking among different compartments. Modulates vesicle budding and uncoating within the Golgi complex. Deactivation induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, suggesting a crucial role in protein trafficking. In its GTP-bound form, its triggers the association with coat proteins with the Golgi membrane. The hydrolysis of ARF1-bound GTP, which is mediated by ARFGAPs proteins, is required for dissociation of coat proteins from Golgi membranes and vesicles.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
  • Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP Ribosylation Factor 1 antibody
    • ADP-ribosylation factor 1 antibody
    • ARF 1 antibody
    • ARF1 antibody
    • ARF1_HUMAN antibody
    see all

References for Anti-ARF1 antibody [ARFS 5F2/2] (ab18109)

ab18109 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (kidney, epithelial cells)
Loading amount 100000 cells
Specification kidney, epithelial cells
Treatment transfected (or not) with ARF1-HA and infected (or not) with influenza A virus
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

Submitted Apr 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney, epithelial cells)
Specification kidney, epithelial cells
Fixative Formaldehyde
Permeabilization Yes - 0.2% triton x100 in PBS
Blocking step FCS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Abcam user community

Verified customer

Submitted Apr 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"