Overview

  • Product nameAnti-ARF3 antibody
    See all ARF3 primary antibodies
  • Description
    Rabbit polyclonal to ARF3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Zebrafish
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 15-79 of Human ARF3 (NP_001650).

  • Positive control
    • 293T, A431, H1299, HeLa, HepG2, MOLT4 or Raji cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab97490 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.

Target

  • FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
  • Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor 3 antibody
    • ADP-ribosylation factor 3 antibody
    • ARF3 antibody
    • ARF3_HUMAN antibody
    • small GTP binding protein antibody
    see all

Anti-ARF3 antibody images

  • Anti-ARF3 antibody (ab97490) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 21 kDa

References for Anti-ARF3 antibody (ab97490)

ab97490 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL60)
Loading amount 10 µg
Specification HL60
Gel Running Conditions Reduced Denaturing (4-20% tris glycine gel)
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
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Submitted Oct 18 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"