Overview

  • Product name
  • Description
    Goat polyclonal to ARF4
  • Host species
    Goat
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Hamster, Cow, Dog, Pig, Xenopus laevis, Zebrafish, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human ARF4 aa 137-150 (Cysteine residue). (NP_001651)
    Sequence:

    SEMTDKLGLQSLRN


    Database link: P18085

  • Positive control
    • Human thymus tissue, HeLa cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab190000 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 19 kDa (predicted molecular weight: 21 kDa).
IHC-P Use a concentration of 5 µg/ml.

Target

Images

  • Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human thymus tissue, labeling ARF4 with ab190000 at 5 µg/ml.

  • Anti-ARF4 antibody (ab190000) at 0.5 µg/ml + HeLa cell lysate at 35 µg

    Developed using the ECL technique.

    Predicted band size: 21 kDa
    Observed band size: 19 kDa (why is the actual band size different from the predicted?)



    Primary incubation was 1 hour.

References

ab190000 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab190000.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up