Recombinant
RabMAb

Anti-ARF4 antibody [EPR12133(B)] (ab171746)

Overview

  • Product name
    Anti-ARF4 antibody [EPR12133(B)]
    See all ARF4 primary antibodies
  • Description
    Rabbit monoclonal [EPR12133(B)] to ARF4
  • Host species
    Rabbit
  • Specificity
    May cross react with family members, immunogen has 86% homology with ARF5
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-P, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Does not react with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human ARF4 aa 50-150 (Cysteine residue). The exact sequence is proprietary.
    Database link: P18085

  • Positive control
    • HT-29, HepG2, K562 and HeLa cell lysates; Human kidney and testis tissues; HepG2 cells; permeabilized K562 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab171746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Predicted molecular weight: 21 kDa.
ICC/IF 1/100 - 1/250.
IHC-P 1/100 - 1/250.
IP 1/10 - 1/100.
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

Target

  • Function
    GTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
  • Sequence similarities
    Belongs to the small GTPase superfamily. Arf family.
  • Cellular localization
    Golgi apparatus.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor 2 antibody
    • ADP ribosylation factor 4 antibody
    • ADP-ribosylation factor 4 antibody
    • ARF2 antibody
    • ARF4 antibody
    • ARF4_HUMAN antibody
    see all

Images

  • All lanes : Anti-ARF4 antibody [EPR12133(B)] (ab171746) at 1/10000 dilution

    Lane 1 : HT-29 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : K562 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 21 kDa

  • Western blot analysis on immunoprecipitation pellet from (Lane 1) HepG2 cell lysate or (Lane 2) 1X PBS (negative control) using ab171746 at 1/10 dilution, and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Immunohistochemistry of paraffin-embedded Human testis tissue labeling ARF4 using ab171746 at 1/100 dilution.

  • Immunohistochemistry of paraffin-embedded Human kidney tissue labeling ARF4 using ab171746 at 1/100 dilution.

  • Immunofluorescence analysis of HepG2 cells labeling ARF4 using ab171746 at 1/100 dilution (green). DAPI nuclear staining (blue).

  • Flow cytometric analysis of permeabilized K562 cells labeling ARF4  using ab171746 (red) at 1/10 dilution, or a rabbit IgG (negative) (green).

References

ab171746 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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