Overview

  • Product nameAnti-ARF5 antibody
    See all ARF5 primary antibodies
  • Description
    Rabbit polyclonal to ARF5
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow
  • Immunogen

    Recombinant protein fragment containing a sequence corresponding to a region within amino acids 12 and 171 of Human ARF5.

  • Positive control
    • WB: Mouse brain and Raji whole cell lysates; 293T cell lysate IHC-P: MDA-MB468 xenograft

Properties

Applications

Our Abpromise guarantee covers the use of ab103974 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.
IHC-P 1/100 - 1/500. Suggested antigen retrieval: use heat mediated 10mM Citrate buffer or Tris-EDTA buffer (pH 8.0).

Target

Anti-ARF5 antibody images

  • Anti-ARF5 antibody (ab103974) at 1/1000 dilution + Raji whole cell lysate at 30 µg

    Predicted band size : 21 kDa
  • Anti-ARF5 antibody (ab103974) at 1/1000 dilution + Mouse brain whole cell lysate at 50 µg

    Predicted band size : 21 kDa
  • ab103974, at a 1/100 dilution, staining ARF5 in paraffin embedded MDA-MB468 xenograft by Immunohistochemical analysis.

References for Anti-ARF5 antibody (ab103974)

ab103974 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HL60)
Loading amount 10 µg
Specification HL60
Gel Running Conditions Reduced Denaturing (15 % Tris-Glycine)
Blocking step Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Oct 22 2012

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (kidney, epithelial cells)
Loading amount 100000 cells
Specification kidney, epithelial cells
Treatment transfected (or not) with ARF5-HA and infected (or not) with influenza A virus
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

Submitted Apr 05 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney, epithelial cells)
Specification kidney, epithelial cells
Fixative Formaldehyde
Permeabilization Yes - 0.2% triton x100 in PBS
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

Submitted Apr 05 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"