Overview

  • Product nameAnti-ARF5 antibody
    See all ARF5 primary antibodies
  • Description
    Rabbit polyclonal to ARF5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 49-113 of Human ARF5

  • Positive control
    • Molt-4, Raji cells lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab96389 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 21 kDa.

Target

  • FunctionGTP-binding protein that functions as an allosteric activator of the cholera toxin catalytic subunit, an ADP-ribosyltransferase. Involved in protein trafficking; may modulate vesicle budding and uncoating within the Golgi apparatus.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Arf family.
  • Cellular localizationGolgi apparatus. Cytoplasm > perinuclear region.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor 5 antibody
    • ADP-ribosylation factor 5 antibody
    • ARF 5 antibody
    • Arf5 antibody
    • ARF5_HUMAN antibody
    see all

Anti-ARF5 antibody images

  • All lanes : Anti-ARF5 antibody (ab96389) at 1/1500 dilution

    Lane 1 : Molt-4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 21 kDa

References for Anti-ARF5 antibody (ab96389)

ab96389 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (kidney, epithelial cells)
Loading amount 100000 cells
Specification kidney, epithelial cells
Treatment transfected (or not) with ARF5-HA and infected (or not) with influenza A virus
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

Submitted Apr 05 2012

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (kidney, epithelial cells)
Specification kidney, epithelial cells
Fixative Formaldehyde
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 18°C
Username

Dr. Maria Amorim

Verified customer

Submitted Apr 03 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"