Overview

  • Product nameAnti-ARF5 antibody [EPR7050]
    See all ARF5 primary antibodies
  • Description
    Rabbit monoclonal [EPR7050] to ARF5
  • Tested applicationsSuitable for: WB, Flow Cytmore details
    Unsuitable for: ICC,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C terminus of Human ARF5.

  • Positive control
    • HeLa and Molt4 cell lysates; HeLa cells
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

Properties

Applications

Our Abpromise guarantee covers the use of ab125023 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
Flow Cyt 1/10 - 1/100. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • Application notesIs unsuitable for ICC,IHC-P or IP.
  • Target

    Anti-ARF5 antibody [EPR7050] images

    • All lanes : Anti-ARF5 antibody [EPR7050] (ab125023) at 1/1000 dilution

      Lane 1 : Hela cell lysate
      Lane 2 : Molt4 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 20 kDa
      Observed band size : 18 kDa (why is the actual band size different from the predicted?)
    • Flow cytometric analysis of permeabilized HeLa cells using ab125023 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-ARF5 antibody [EPR7050] (ab125023)

    ab125023 has not yet been referenced specifically in any publications.

    Product Wall

    There are currently no Abreviews or Questions for ab125023.
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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"