Anti-HUWE1/Mule antibody (ab65153)
Key features and details
- Rabbit polyclonal to HUWE1/Mule
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HUWE1/Mule antibody
See all HUWE1/Mule primary antibodies -
Description
Rabbit polyclonal to HUWE1/Mule -
Host species
Rabbit -
Specificity
This antibody reacts with HUWE1/Mule.
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Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Rat, Human -
Immunogen
Synthetic peptide corresponding to HUWE1/Mule (C terminal). Peptide corresponding to 19 amino acids near the C-terminus of human HUWE1.
Database link: Q7Z6Z7 -
Positive control
- Brain, cortex; heart.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 1 µg/ml.
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 481 kDa.
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IHC-P |
Use a concentration of 5 µg/ml.
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Notes |
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ICC/IF
Use a concentration of 1 µg/ml. |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 481 kDa. |
IHC-P
Use a concentration of 5 µg/ml. |
Target
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Function
E3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation. -
Tissue specificity
Weakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas. -
Pathway
Protein modification; protein ubiquitination. -
Involvement in disease
Defects in HUWE1 are the cause of mental retardation syndromic X-linked Turner type (MRXST) [MIM:300706]; also known as mental retardation and macrocephaly syndrome. MRXST shows clinical variability. Associated phenotypes include macrocephaly and variable contractures.
A chromosomal microduplication involving HUWE1 and HSD17B10 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation. -
Sequence similarities
Belongs to the TOM1/PTR1 family.
Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
Contains 1 UBA domain.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 WWE domain. -
Domain
The HECT domain mediates inhibition of the transcriptional activity of p53. -
Post-translational
modificationsPhosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.
Phosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm. Nucleus. Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells. - Information by UniProt
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Database links
- Entrez Gene: 10075 Human
- SwissProt: Q7Z6Z7 Human
- SwissProt: P51593 Rat
- Unigene: 136905 Human
- Unigene: 12130 Rat
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Alternative names
- ARF binding protein 1 antibody
- ARF BP1 antibody
- ARF-binding protein 1 antibody
see all
Images
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Western blot analysis using ab65153 in Daudi cell lysate at 1 ug/ml
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ab65153 at 5µg/ml staining HUWE1/Mule in human Brain, cortex tissue.
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Immunohistochemistry analysis of paraffin embedded formalin fixed human heart tissue using 5 µg/ml ab65153.
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ICC/IF image of ab65153 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab65153 has not yet been referenced specifically in any publications.