Overview

  • Product nameAnti-ARFBP1 antibody
    See all ARFBP1 primary antibodies
  • Description
    Rabbit polyclonal to ARFBP1
  • SpecificityThis antibody reacts with ARFBP1
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to ARFBP1 (C terminal). Peptide corresponding to 19 amino acids near the C-terminus of human ARFBP1.
    Database link: Q7Z6Z7

  • Positive control
    • Brain, cortex; heart.

Properties

Applications

Our Abpromise guarantee covers the use of ab65153 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 481 kDa.
IHC-P Use a concentration of 5 µg/ml.

Target

  • FunctionE3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.
  • Tissue specificityWeakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in HUWE1 are the cause of mental retardation syndromic X-linked Turner type (MRXST) [MIM:300706]; also known as mental retardation and macrocephaly syndrome. MRXST shows clinical variability. Associated phenotypes include macrocephaly and variable contractures.
    A chromosomal microduplication involving HUWE1 and HSD17B10 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
  • Sequence similaritiesBelongs to the TOM1/PTR1 family.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 UBA domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 WWE domain.
  • DomainThe HECT domain mediates inhibition of the transcriptional activity of p53.
  • Post-translational
    modifications
    Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARF binding protein 1 antibody
    • ARF BP1 antibody
    • ARF-binding protein 1 antibody
    • ARF-BP1 antibody
    • BJ-HCC-24 tumor antigen antibody
    • E3 ubiquitin protein ligase HUWE1 antibody
    • E3 ubiquitin-protein ligase HUWE1 antibody
    • HECT antibody
    • HECT domain protein LASU1 antibody
    • HECT UBA and WWE domain containing protein 1 antibody
    • HectH9 antibody
    • Homologous to E6AP carboxyl terminus homologous protein 9 antibody
    • Huwe1 antibody
    • HUWE1_HUMAN antibody
    • Ib772 antibody
    • Large structure of UREB1 antibody
    • LASU1 antibody
    • Mcl 1 ubiquitin ligase E3 antibody
    • Mcl-1 ubiquitin ligase E3 antibody
    • Mule antibody
    • UBA and WWE domain-containing protein 1 antibody
    • Upstream regulatory element-binding protein 1 antibody
    • URE-B1 antibody
    • URE-binding protein 1 antibody
    • UREB1 antibody
    see all

Anti-ARFBP1 antibody images



  • Predicted band size : 481 kDa

    Western blot analysis using ab65153 in Daudi cell lysate at 1 ug/ml 

  • ab65153 at 5µg/ml staining ARFBP1 in Brain, cortex tissue.
  • Immunohistochemistry analysis of paraffin embedded formalin fixed heart tissue using 5 µg/ml ab65153.
  • ICC/IF image of ab65153 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab65153, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-ARFBP1 antibody (ab65153)

ab65153 has not yet been referenced specifically in any publications.

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