• Product nameAnti-ARFBP1 antibody
    See all ARFBP1 primary antibodies
  • Description
    Rabbit polyclonal to ARFBP1
  • Tested applicationsSuitable for: ICC/IF, WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Gorilla, Orangutan
  • Immunogen

    A region between residues 2250 and 2300 of human ARFBP1 (NP_113584.3)

  • Positive control
    • Whole cell lysate from 293T cells.



Our Abpromise guarantee covers the use of ab70161 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 µg/ml.
WB 1/1000 - 1/5000. Detects a band of approximately >460 kDa (predicted molecular weight: 481 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.


  • FunctionE3 ubiquitin-protein ligase which mediates ubiquitination and subsequent proteasomal degradation of target proteins. Regulates apoptosis by catalyzing the polyubiquitination and degradation of MCL1. Mediates monoubiquitination of DNA polymerase beta (POLB) at 'Lys-41', 'Lys-61' and 'Lys-81', thereby playing a role in base-excision repair. Also ubiquitinates the p53/TP53 tumor suppressor and core histones including H1, H2A, H2B, H3 and H4. Binds to an upstream initiator-like sequence in the preprodynorphin gene. Regulates neural differentiation and proliferation by catalyzing the polyubiquitination and degradation of MYCN. May regulate abundance of CDC6 after DNA damage by polyubiquitinating and targeting CDC6 to degradation.
  • Tissue specificityWeakly expressed in heart, brain and placenta but not in other tissues. Expressed in a number of cell lines, predominantly in those from colorectal carcinomas.
  • PathwayProtein modification; protein ubiquitination.
  • Involvement in diseaseDefects in HUWE1 are the cause of mental retardation syndromic X-linked Turner type (MRXST) [MIM:300706]; also known as mental retardation and macrocephaly syndrome. MRXST shows clinical variability. Associated phenotypes include macrocephaly and variable contractures.
    A chromosomal microduplication involving HUWE1 and HSD17B10 is the cause of mental retardation X-linked type 17 (MRX17) [MIM:300705]; also known as mental retardation X-linked type 31 (MRX31). Mental retardation is characterized by significantly sub-average general intellectual functioning associated with impairments in adaptative behavior and manifested during the developmental period. In contrast to syndromic or specific X-linked mental retardation which also present with associated physical, neurological and/or psychiatric manifestations, intellectual deficiency is the only primary symptom of non-syndromic X-linked mental retardation.
  • Sequence similaritiesBelongs to the TOM1/PTR1 family.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 1 UBA domain.
    Contains 1 UIM (ubiquitin-interacting motif) repeat.
    Contains 1 WWE domain.
  • DomainThe HECT domain mediates inhibition of the transcriptional activity of p53.
  • Post-translational
    Phosphorylated on tyrosine; phosphorylation is probably required for its ability to inhibit TP53 transactivation.
    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Nucleus. Mainly expressed in the cytoplasm of most tissues, except in the nucleus of spermatogonia, primary spermatocytes and neuronal cells (By similarity). Predominantly cytosolic or perinuclear in some colorectal carcinoma cells.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARF binding protein 1 antibody
    • ARF BP1 antibody
    • ARF-binding protein 1 antibody
    • ARF-BP1 antibody
    • BJ-HCC-24 tumor antigen antibody
    • E3 ubiquitin protein ligase HUWE1 antibody
    • E3 ubiquitin-protein ligase HUWE1 antibody
    • HECT antibody
    • HECT domain protein LASU1 antibody
    • HECT UBA and WWE domain containing protein 1 antibody
    • HectH9 antibody
    • Homologous to E6AP carboxyl terminus homologous protein 9 antibody
    • Huwe1 antibody
    • HUWE1_HUMAN antibody
    • Ib772 antibody
    • Large structure of UREB1 antibody
    • LASU1 antibody
    • Mcl 1 ubiquitin ligase E3 antibody
    • Mcl-1 ubiquitin ligase E3 antibody
    • Mule antibody
    • UBA and WWE domain-containing protein 1 antibody
    • Upstream regulatory element-binding protein 1 antibody
    • URE-B1 antibody
    • URE-binding protein 1 antibody
    • UREB1 antibody
    see all

Anti-ARFBP1 antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue labelling ARFBP1 with ab70161 at 1/1000 (1µg/ml). Detection: DAB.
  • All lanes : Anti-ARFBP1 antibody (ab70161) at 1 µg/ml

    Lane 1 : 293T whole cell lysate at 50 µg
    Lane 2 : 293T whole cell lysate at 15 µg
    Lane 3 : 293T whole cell lysate at 5 µg

    Predicted band size : 481 kDa
    Observed band size : >460 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 200 kDa,300 kDa. We are unsure as to the identity of these extra bands.
  • ICC/IF image of ab70161 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • IHC image of ab70161 staining in human normal heart formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with EDTA (pH9, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab70161, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-ARFBP1 antibody (ab70161)

This product has been referenced in:
  • Horikawa I  et al. Autophagic degradation of the inhibitory p53 isoform ?133p53a as a regulatory mechanism for p53-mediated senescence. Nat Commun 5:4706 (2014). Read more (PubMed: 25144556) »
  • Izumi H & Kaneko Y Trim32 facilitates degradation of MYCN on spindle poles and induces asymmetric cell division in human neuroblastoma cells. Cancer Res 74:5620-30 (2014). Read more (PubMed: 25100564) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Reduced Denaturing (10%)
Sample Human Cell lysate - whole cell (HEK293)
Specification HEK293
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 29 2014

Thank you for your email.
I have added a vial of ab70161 in Order 1086585. It wasn't possible to add the vial in the current "dispatch today" order.
I hope this informaiton will be helpful.
Have a good weekend!

Thank you for taking time to complete our questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.
The details provided will enable us to investigate this case and will provide us with vital info...

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Thank you for your email. I am sorry to hear that you are experiencing problems with this antibody.
I have checked the protocol and images you have kindly sent I however have few following questions:
- The molecular weight of ARFBP1 is ˜400 k...

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