Anti-ARFGAP1 antibody - C-terminal (ab71759)

Overview

  • Product nameAnti-ARFGAP1 antibody - C-terminal
    See all ARFGAP1 primary antibodies
  • Description
    Rabbit polyclonal to ARFGAP1 - C-terminal
  • Tested applicationsSuitable for: ELISA, IHC-P, WBmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide from the C-terminal region of human ARFGAP1 conjugated to KLH

  • Positive control
    • Rat recombinant ARFGAP1. Human breast carcinoma tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab71759 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 45 kDa).

Target

  • FunctionGTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and RNP24. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine.
  • Sequence similaritiesContains 1 Arf-GAP domain.
  • DomainThe region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes.
  • Cellular localizationCytoplasm. Golgi apparatus. Associates with the Golgi complex.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor 1 GTPase activating protein antibody
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP-ribosylation factor 1 GTPase-activating protein antibody
    • ADP-ribosylation factor GTPase-activating protein 1 antibody
    • ARF GAP 1 antibody
    • ARF1 directed GTPase activating protein antibody
    • ARF1 GAP antibody
    • ARF1-directed GTPase-activating protein antibody
    • ARF1GAP antibody
    • ARFG1_HUMAN antibody
    • Arfgap1 antibody
    • GAP protein antibody
    • HRIHFB2281 antibody
    see all

Anti-ARFGAP1 antibody - C-terminal images

  • All lanes : Anti-ARFGAP1 antibody - C-terminal (ab71759) at 1/100 dilution

    Lane 1 : rat recombinant ARFGAP1 at 0.03 µg
    Lane 2 : rat recombinant ARFGAP1 at 0.01 µg
    Lane 3 : rat recombinant ARFGAP1 at 0.003 µg


    Predicted band size : 45 kDa
    Observed band size : 50 kDa (why is the actual band size different from the predicted?)
  • ab71759 at 1/50 dilution, staining ARFGAP1 in human breast carcinoma by Immunohistochemistry using formalin-fixed, paraffin-embedded tissue, followed by peroxidase-conjugated secondary antibody and AEC staining.

References for Anti-ARFGAP1 antibody - C-terminal (ab71759)

ab71759 has not yet been referenced specifically in any publications.

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