Overview

  • Product name
  • Description
    Goat polyclonal to ARFGAP3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Dog, Human
  • Immunogen

    Synthetic peptide:

    C-NGVVTSIQDRYGS

    , corresponding to C terminal amino acids 504-516 of Human ARFGAP3 (Peptide available as ab22801.)

  • General notes
    Principal Names: ARFGAP1, ADP ribosylation factor GTPase activating protein 1, ARFGAP3. GenBank Accession Number: NP055385 LocusLink ID: 26286


    ARFs are critical in vesicular trafficking pathway.

Properties

Applications

Our Abpromise guarantee covers the use of ab1308 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Can be blocked with Human ARFGAP3 peptide (ab22801).

Approx 55-60kDa band seen in Mouse Brain extracts.

Target

  • Function
    GTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
  • Tissue specificity
    Widely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
  • Sequence similarities
    Contains 1 Arf-GAP domain.
  • Developmental stage
    Expressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
  • Cellular localization
    Cytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP ribosylation factor GTPase activating protein 3 antibody
    • ADP-ribosylation factor GTPase-activating protein 3 antibody
    • ARF GAP 3 antibody
    • ARFG3_HUMAN antibody
    • ArfGAP 3 antibody
    • ARFGAP1 antibody
    • ARFGAP3 antibody
    • FLJ45618 antibody
    see all

Images

  • ARFGAP3 staining (2µg/ml) of Mouse Brain extracts (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence

References

ab1308 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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