Overview

  • Product nameAnti-ARFGAP3 antibody
    See all ARFGAP3 primary antibodies
  • Description
    Goat polyclonal to ARFGAP3
  • SpecificityAb27959 recognises ARFGAP1.
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chicken, Cynomolgus Monkey
  • Immunogen

    Synthetic peptide:

    NGVVTSIQDRYGS

    , corresponding to C terminal amino acids 504-516 of Human ARFGAP1.

  • Positive control
    • Mouse brain extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab27959 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 44 kDa.

Target

  • FunctionGTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
  • Tissue specificityWidely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
  • Sequence similaritiesContains 1 Arf-GAP domain.
  • Developmental stageExpressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
  • Cellular localizationCytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
  • Information by UniProt
  • Database links
  • Alternative names
    • ADP ribosylation factor GTPase activating protein 1 antibody
    • ADP ribosylation factor GTPase activating protein 3 antibody
    • ADP-ribosylation factor GTPase-activating protein 3 antibody
    • ARF GAP 3 antibody
    • ARFG3_HUMAN antibody
    • ArfGAP 3 antibody
    • ARFGAP1 antibody
    • ARFGAP3 antibody
    • FLJ45618 antibody
    see all

Anti-ARFGAP3 antibody images

  • All lanes : Anti-ARFGAP3 antibody (ab27959) at 1/1000 dilution

    Lane 1 : 50000 non-transfected HeLa cells.
    Lane 2 : 50000 HeLa cells expressing closely related ArfGAP2 (zfp289) in the pPCMUIV vector.
    Lane 3 : 50000 HeLa cells transfected with full length ArfGAP3 in the pPCMUIV vector.

    Secondary
    HRP conjugated rabbit anti-goat IgG
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 30 seconds

    This image is courtesy of an Abreview submitted by Mr Fredrik Kartberg

    See Abreview

References for Anti-ARFGAP3 antibody (ab27959)

ab27959 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HeLa cells)
Loading amount 50000 cells
Specification HeLa cells
Blocking step Milk as blocking agent for 15 minute(s) · Concentration: 5%
Username

Mr. Fredrik Kartberg

Verified customer

Submitted Sep 13 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"