The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 44 kDa.
FunctionGTPase-activating protein (GAP) for ADP ribosylation factor 1 (ARF1). Hydrolysis of ARF1-bound GTP may lead to dissociation of coatomer from Golgi-derived membranes to allow fusion with target membranes.
Tissue specificityWidely expressed. Highest expression in endocrine glands (pancreas, pituitary gland, salivary gland, and prostate) and testis with a much higher expression in the testis than in the ovary.
Sequence similaritiesContains 1 Arf-GAP domain.
Developmental stageExpressed at higher level in adult thymus, brain and lung, than in corresponding fetal tissues. Expressed at lower level in spleen, heart, kidney and liver during development.
Cellular localizationCytoplasm. Golgi apparatus membrane. Also found on peripheral punctate structures likely to be endoplasmic reticulum-Golgi intermediate compartment.
ADP ribosylation factor GTPase activating protein 1 antibody
ADP ribosylation factor GTPase activating protein 3 antibody
ADP-ribosylation factor GTPase-activating protein 3 antibody
ARF GAP 3 antibody
ArfGAP 3 antibody
Anti-ARFGAP3 antibody images
Western blot - ARFGAP3 antibody (ab27959)
All lanes : Anti-ARFGAP3 antibody (ab27959) at 1/1000 dilution
Lane 1 : 50000 non-transfected HeLa cells. Lane 2 : 50000 HeLa cells expressing closely related ArfGAP2 (zfp289) in the pPCMUIV vector. Lane 3 : 50000 HeLa cells transfected with full length ArfGAP3 in the pPCMUIV vector.
Secondary HRP conjugated rabbit anti-goat IgG developed using the ECL technique