Overview

  • Product nameAnti-ARFGEF2 antibody
    See all ARFGEF2 primary antibodies
  • Description
    Rabbit polyclonal to ARFGEF2
  • Specificityab75001 detects endogenous levels of total ARFGEF2 protein.
  • Tested applicationsSuitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human ARFGEF2.

  • Positive control
    • A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 202 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • RelevanceADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.
  • Cellular localizationCytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
  • Database links
  • Alternative names
    • ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
    • ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
    • ARFGEF 2 antibody
    • ARFGEF2 antibody
    • ARFGEP2 antibody
    • BIG 2 antibody
    • BIG2 antibody
    • Brefeldin A inhibited 2 antibody
    • Brefeldin A inhibited GEP 2 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 2 antibody
    • dJ1164I10.1 antibody
    • FLJ23723 antibody
    see all

Anti-ARFGEF2 antibody images

  • All lanes : Anti-ARFGEF2 antibody (ab75001) at 1/500 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 202 kDa
    Observed band size : 202 kDa
    Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of ARFGEF2 in A549 cells, using ab75001 at 1/500 dilution. Right panel was also treated with immunising peptide.

References for Anti-ARFGEF2 antibody (ab75001)

ab75001 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab75001.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"