Overview

  • Product name
  • Description
    Rabbit polyclonal to ARFGEF2
  • Specificity
    ab75001 detects endogenous levels of total ARFGEF2 protein.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from an internal region of human ARFGEF2.

  • Positive control
    • A549 cells

Properties

Applications

Our Abpromise guarantee covers the use of ab75001 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 202 kDa.
ELISA 1/20000.
ICC/IF 1/500 - 1/1000.

Target

  • Relevance
    ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.
  • Cellular localization
    Cytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
  • Database links
  • Alternative names
    • ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
    • ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
    • ARFGEF 2 antibody
    • ARFGEF2 antibody
    • ARFGEP2 antibody
    • BIG 2 antibody
    • BIG2 antibody
    • Brefeldin A inhibited 2 antibody
    • Brefeldin A inhibited GEP 2 antibody
    • Brefeldin A inhibited guanine nucleotide exchange protein 2 antibody
    • dJ1164I10.1 antibody
    • FLJ23723 antibody
    see all

Images

  • All lanes : Anti-ARFGEF2 antibody (ab75001) at 1/500 dilution

    Lane 1 : A549 cell extract
    Lane 2 : A549 cell extract with immunising peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 202 kDa
    Observed band size : 202 kDa
    Additional bands at : 125 kDa. We are unsure as to the identity of these extra bands.
  • Immunofluorescence analysis of ARFGEF2 in A549 cells, using ab75001 at 1/500 dilution. Right panel was also treated with immunising peptide.

References

ab75001 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab75001.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up