Recombinant
RabMAb

Anti-ARFGEF2 antibody [EPR10049] (ab180186)

Overview

  • Product name
    Anti-ARFGEF2 antibody [EPR10049]
    See all ARFGEF2 primary antibodies
  • Description
    Rabbit monoclonal [EPR10049] to ARFGEF2
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human ARFGEF2 aa 1500-1600 (Cysteine residue). The exact sequence is proprietary.
    Database link: Q9Y6D5

  • Positive control
    • HeLa, HepG2 and 293T cell lysates.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab180186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 202 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-P or IP.
  • Target

    • Relevance
      ADP-ribosylation factors (ARFs) play an important role in intracellular vesicular trafficking. ARFGEF2 promotes guanine-nucleotide exchange on ARF1, ARF5 and ARF6 and the activation of ARF1/ARF5/ARF6 through replacement of GDP with GTP. It contains a Sec7 domain, which may be responsible for its guanine-nucleotide exchange activity and also brefeldin A inhibition.
    • Cellular localization
      Cytoplasm. Membrane. Golgi apparatus. Cytoplasm › perinuclear region. Golgi apparatus › trans-Golgi network By similarity. Endosome By similarity. Cytoplasm › cytoskeleton › microtubule organizing center › centrosome. Cell projection › dendrite By similarity. Cytoplasmic vesicle By similarity. Cell junction › synapse By similarity. Cytoplasm › cytoskeleton By similarity. Note: Translocates from cytoplasm to membranes upon cAMP treatment. Localized in recycling endosomes.
    • Database links
    • Alternative names
      • ADP ribosylation factor guanine nucleotide exchange factor 2 (brefeldin A inhibited) antibody
      • ADP ribosylation factor guanine nucleotide exchange factor 2 antibody
      • ARFGEF 2 antibody
      • ARFGEF2 antibody
      • ARFGEP2 antibody
      • BIG 2 antibody
      • BIG2 antibody
      • Brefeldin A inhibited 2 antibody
      • Brefeldin A inhibited GEP 2 antibody
      • Brefeldin A inhibited guanine nucleotide exchange protein 2 antibody
      • dJ1164I10.1 antibody
      • FLJ23723 antibody
      see all

    Images

    • All lanes : Anti-ARFGEF2 antibody [EPR10049] (ab180186) at 1/1000 dilution

      Lane 1 : HeLa cell lysate
      Lane 2 : HepG2 cell lysate
      Lane 3 : 293T cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 202 kDa

    References

    ab180186 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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