Overview

  • Product nameAnti-Arg 3.1 antibody
    See all Arg 3.1 primary antibodies
  • Description
    Rabbit polyclonal to Arg 3.1
  • Tested applicationsSuitable for: IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Rat Arg3.1.

    (Peptide available as ab26899.)

  • Positive control
    • This antibody gave a positive signal in the following tissue lysates: Mouse forebrain, Mouse hippocampus, Rat cortex, Rat hippocampus.

Properties

Applications

Our Abpromise guarantee covers the use of ab23382 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/300.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 45 kDa).

Abcam recommends using 3% milk as the blocking agent.

Target

  • FunctionRequired for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors (By similarity). Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration.
  • Sequence similaritiesBelongs to the ARC/ARG3.1 family.
  • Cellular localizationCytoplasm, cytoskeleton. Endosome. Cytoplasmic vesicle, secretory vesicle, acrosome. Cell junction, synapse, postsynaptic cell membrane, postsynaptic density. Cell projection, dendrite. Cell projection, dendritic spine. Cell junction, synapse. Associated with the cell cortex of neuronal soma and dendrites. Enriched in postsynaptic density of dendritic spines. Associated with the sperm tail (By similarity). Enriched on the plasma membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Activity regulated cytoskeletal associated protein antibody
    • Activity regulated cytoskeletal protein antibody
    • Activity regulated gene 3.1 protein homolog antibody
    • Activity-regulated cytoskeleton-associated protein antibody
    • Activity-regulated gene 3.1 protein homolog antibody
    • Arc antibody
    • ARC/ARG3.1 antibody
    • ARC_HUMAN antibody
    • Arg3.1 antibody
    • KIAA0278 antibody
    see all

Anti-Arg 3.1 antibody images

  • All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/ml (with 3% milk block)

    Lane 1 : Forebrain (Mouse) Tissue Lysate
    Lane 2 : Rat Cortex Tissue Lysate
    Lane 3 : Hippocampus (Mouse) Tissue Lysate
    Lane 4 : Rat Hippocampus Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 12 minutes
  • All lanes : Anti-Arg 3.1 antibody (ab23382) at 1 µg/ml

    Lane 1 : Hippocampus (Mouse) Tissue Lysate blocked with 5% BSA
    Lane 2 : Rat Hippocampus Tissue Lysate blocked with 5% BSA
    Lane 3 : Hippocampus (Mouse) Tissue Lysate blocked with 5% milk
    Lane 4 : Rat Hippocampus Tissue Lysate blocked with 5% milk
    Lane 5 : Hippocampus (Mouse) Tissue Lysate blocked with 3% milk
    Lane 6 : Rat Hippocampus Tissue Lysate blocked with 3% milk
    Lane 7 : Hippocampus (Mouse) Tissue Lysate blocked with 1% milk
    Lane 8 : Rat Hippocampus Tissue Lysate blocked with 1% milk

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 20 minutes

References for Anti-Arg 3.1 antibody (ab23382)

This product has been referenced in:
  • Wu H  et al. Salicylate-induced changes in immediate-early genes in the hippocampal CA1 area. Mol Med Rep 12:1625-30 (2015). WB, IHC-P ; Rat . Read more (PubMed: 25873216) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (8% Bis-Tris in MES)
Sample Mouse Tissue lysate - other (Hippocampal Cytoplasm)
Specification Hippocampal Cytoplasm
Blocking step Li-Cor Block Buffer as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 21°C
Username

Dr. Grant Corbett

Verified customer

Submitted Jul 23 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Username

Dr. Sophie Pezet

Verified customer

Submitted Dec 10 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (e14.5 striatum)
Specification e14.5 striatum
Fixative Paraformaldehyde
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Dean Griffiths

Verified customer

Submitted Aug 17 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"