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Synthetic peptide: LDLPQKQGEP LDQFLWRKRD LYQTLYVDAD EEEIIQYVVG TLQPKLKRFL , corresponding to internal sequence amino acids 288-337 of Human Arg 3.1 (NP_056008).
Our Abpromise guarantee covers the use of ab85656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 45 kDa.Can be blocked with Arg 3.1 peptide (ab117435). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adrenal tissue labelling Arg 3.1 with ab85656 at 5µg/ml.