Overview

  • Product name
  • Description
    Rabbit polyclonal to Arg2
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Pig
  • Immunogen

    Synthetic peptide from within the C termina amino acids 289-338 (SALDLVEVNP QLATSEEEAK TTANLAVDVI ASSFGQTREG GHIVYDQLPT) of Human Arg2 (NP_001163)

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab81505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • Function
    May play a role in the regulation of extra-urea cycle arginine metabolism and also in down-regulation of nitric oxide synthesis. Extrahepatic arginase functions to regulate L-arginine bioavailability to NO synthase. Since NO synthase is found in the penile corpus cavernosum smooth muscle, the clitoral corpus cavernosum and the vagina, arginase II plays a role in both male and female sexual arousal. It is therefore a potential target for the treatment of male and female sexual arousal disorders.
  • Tissue specificity
    Expressed most strongly in kidney and prostate, much less strongly in the brain, skeletal muscle, placenta, lung, mammary gland, macrophage, uterus, testis and gut, but apparently not in the liver, heart and pancreas.
  • Pathway
    Nitrogen metabolism; urea cycle; L-ornithine and urea from L-arginine: step 1/1.
  • Sequence similarities
    Belongs to the arginase family.
  • Cellular localization
    Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • ARG2 antibody
    • ARGI2_HUMAN antibody
    • Arginase II mitochondrial antibody
    • Arginase type II antibody
    • Arginase-2 antibody
    • arginase-2, mitochondrial antibody
    • Kidney arginase antibody
    • Kidney type arginase antibody
    • Kidney-type arginase antibody
    • L arginine amidinohydrolase antibody
    • L arginine ureahydrolase antibody
    • mitochondrial antibody
    • Non hepatic arginase antibody
    • Non-hepatic arginase antibody
    • Nonhepatic arginase antibody
    • Type II arginase antibody
    see all

Images

  • Anti-Arg2 antibody (ab81505) at 1 µg/ml + Jurkat cell lysate at 10 µg with skim milk/ PBS buffer at 5 %

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

References

This product has been referenced in:
  • Bichell TJV  et al. Reduced bioavailable manganese causes striatal urea cycle pathology in Huntington's disease mouse model. Biochim Biophys Acta 1863:1596-1604 (2017). Read more (PubMed: 28213125) »
  • Elahi S  et al. Immunosuppressive CD71+ erythroid cells compromise neonatal host defence against infection. Nature 504:158-62 (2013). Read more (PubMed: 24196717) »

See all 3 Publications for this product

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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