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Arginase Activity Assay (Colorimetric) (ab180877) kit is a simple, sensitive and rapid assay to quantify arginase activity. Arginase reacts with arginine and undergoes a series of reactions that form an intermediate that react stoichiometrically with the probe to generate a colored product that can detected at OD = 570 nm.
The kit can detect less than 0.2 U/L Arginase activity in 96-well assay format.
Arginase (EC 126.96.36.199) is the final enzyme of the Urea Cycle. It converts L-arginine into urea and L-ornithine and plays an important role in removing ammonium ion from the body. Arginase has two isoforms: Arginase I and Arginase II. Arginase I is mainly present in the liver and plays an important part in the urea cycle whereas Arginase II is present in kidney and other tissues and regulates Arginine/Ornithine concentration. Arginase deficiency can lead to severe symptoms including neurological impairment, dementia and hyperammonemia. Analysis of Arginase activity is fundamental to the study of the urea metabolic pathway.
|Arginase Assay Buffer||WM||1 x 25ml|
|Arginase Converter Enzyme (Lyophilized)||Blue||1 vial|
|Arginase Developer (Lyophilized)||Orange||1 vial|
|Arginase Enzyme Mix (Lyophilized)||Green||1 vial|
|Arginase Positive Control (Lyophilized)||Purple||1 vial|
|Arginase Substrate (Lyophilized)||Clear||1 vial|
|Hydrogen peroxide||Yellow||1 x 100µl|
|OxiRed Probe in DMSO||Red||1 x 200µl|
Our Abpromise guarantee covers the use of ab180877 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"