Anti-Argininosuccinate Lyase antibody (ab97370)

Overview

  • Product nameAnti-Argininosuccinate Lyase antibody
    See all Argininosuccinate Lyase primary antibodies
  • Description
    Rabbit polyclonal to Argininosuccinate Lyase
  • Tested applicationsSuitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Recombinant protein fragment corresponding to a region within amino acids 13 - 231 of Human Argininosuccinate Lyase (AAH08195).

  • Positive control
    • WB: 293T, A431, H1299, HeLa, HepG2 and MOLT4 cell lysates IF: HeLa cells

Properties

Applications

Our Abpromise guarantee covers the use of ab97370 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 52 kDa.
ICC/IF 1/100 - 1/200.

Target

  • PathwayAmino-acid biosynthesis; L-arginine biosynthesis; L-arginine from L-ornithine and carbamoyl phosphate: step 3/3.
    Nitrogen metabolism; urea cycle; L-arginine and fumarate from (N(omega)-L-arginino)succinate: step 1/1.
  • Involvement in diseaseDefects in ASL are the cause of arginosuccinicaciduria (ARGINSA) [MIM:207900]. Arginosuccinicaciduria is an autosomal recessive disorder of the urea cycle. The disease is characterized by mental and physical retardation, liver enlargement, skin lesions, dry and brittle hair showing trichorrhexis nodosa microscopically and fluorescing red, convulsions, and episodic unconsciousness.
  • Sequence similaritiesBelongs to the lyase 1 family. Argininosuccinate lyase subfamily.
  • Post-translational
    modifications
    Acetylation modifies enzyme activity in response to alterations of extracellular nutrient availability. Acetylation increased with trichostin A (TSA) or with nicotinamide (NAM). Glucose increases acetylation by about a factor of 3 with decreasing enzyme activity. Acetylation on Lys-288 is decreased on the addition of extra amino acids resulting in activation of enzyme activity.
  • Information by UniProt
  • Database links
  • Alternative names
    • Argininosuccinase antibody
    • Argininosuccinate lyase antibody
    • Arginosuccinase antibody
    • ARLY_HUMAN antibody
    • ASAL antibody
    • ASL antibody
    • EC 4.3.2.1 antibody
    see all

Anti-Argininosuccinate Lyase antibody images

  • All lanes : Anti-Argininosuccinate Lyase antibody (ab97370) at 1/1000 dilution

    Lane 1 : HeLa whole cell lysate
    Lane 2 : HepG2 whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 52 kDa
  • ab97370, at a 1/200 dilution, staining Argininosuccinate Lyase in paraformaldehyde-fixed HeLa cells by Immunofluorescence analysis. Right image is merged with a DNA probe.

References for Anti-Argininosuccinate Lyase antibody (ab97370)

ab97370 has not yet been referenced specifically in any publications.

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (A375)
Loading amount 20 µg
Specification A375
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
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Submitted Jan 21 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"