Overview

  • Product nameAnti-Arginyl tRNA synthetase antibody
    See all Arginyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Arginyl tRNA synthetase
  • SpecificityThis antibody reacts specifically with 74kDa RRS and immunoprecipitates with AIMP1(p43).
  • Tested applicationsSuitable for: IHC-P, WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Native recombinant corresponding to amino acids 1-72 of Human RRS.

  • Positive control
    • HEK293 cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab31537 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/50 - 1/100.
WB 1/3000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
ICC/IF 1/4000 - 1/8000.
IP 1/4000 - 1/8000.

Target

  • FunctionForms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
  • Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
  • DomainThe N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.
  • Cellular localizationCytoplasm.
  • Information by UniProt
  • Database links
  • FormThere are 2 isoforms produced by alternative initiation.
  • Alternative names
    • 2610011N19Rik antibody
    • 2610037E21Rik antibody
    • AL033339 antibody
    • Arginine tRNA ligase 1, cytoplasmic antibody
    • Arginine tRNA ligase antibody
    • Arginine--tRNA ligase antibody
    • Arginyl tRNA synthetase 1 antibody
    • Arginyl tRNA synthetase cytoplasmic antibody
    • Arginyl tRNA synthetase, cytoplasmic antibody
    • Arginyl-tRNA synthetase, cytoplasmic antibody
    • ArgRS antibody
    • AW985894 antibody
    • DALRD 1 antibody
    • DALRD1 antibody
    • EC 6.1.1.19 antibody
    • MGC8641 antibody
    • MGC86730 antibody
    • RARS antibody
    • SYRC_HUMAN antibody
    • wu:fa96g09 antibody
    • zgc:56050 antibody
    • zgc:86730 antibody
    see all

Anti-Arginyl tRNA synthetase antibody images

  • Anti-Arginyl tRNA synthetase antibody (ab31537) at 1/4000 dilution + HEK293 cell lysate

    Predicted band size : 74 kDa
    Observed band size : 74 kDa
  • Immunofluorescence of a Chang cell using ab31537 at a dilution of 1/1000.
  • Formalin fixed-paraffin embedded Immunohistochemistry staining of Human breast tissue using ab31537 at 1/4000.
  • Anti-Arginyl tRNA synthetase antibody (ab31537) at 1/3000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 74 kDa
    Observed band size : 74 kDa

References for Anti-Arginyl tRNA synthetase antibody (ab31537)

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Zhang X  et al. Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures. Am J Hum Genet 94:547-58 (2014). Read more (PubMed: 24656866) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hepatocarcinoma)
Loading amount 100 µg
Specification Hepatocarcinoma
Gel Running Conditions Reduced Denaturing (4-12%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Silvia Marquez Jurado

Verified customer

Submitted Sep 26 2011

Application Immunocytochemistry/ Immunofluorescence
Sample Pig Cell (Swine Cell)
Specification Swine Cell
Fixative Formaldehyde
Permeabilization Yes - Triton-100 05% in PBS 1X
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: RT°C
Username

Ms. Silvia Marquez Jurado

Verified customer

Submitted Sep 14 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"