The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/50 - 1/100.
1/3000. Detects a band of approximately 74 kDa (predicted molecular weight: 74 kDa).
1/4000 - 1/8000.
1/4000 - 1/8000.
FunctionForms part of a macromolecular complex that catalyzes the attachment of specific amino acids to cognate tRNAs during protein synthesis. Modulates the secretion of AIMP1 and may be involved in generation of the inflammatory cytokine EMAP2 from AIMP1.
Sequence similaritiesBelongs to the class-I aminoacyl-tRNA synthetase family.
DomainThe N-terminus (AA 1-72) has two regions predicted to be alpha-helical that might be involved in the multisynthetase complex assembly.
Formalin fixed-paraffin embedded Immunohistochemistry staining of Human breast tissue using ab31537 at 1/4000.
Western blot - Arginyl tRNA synthetase antibody (ab31537)
Anti-Arginyl tRNA synthetase antibody (ab31537) at 1/3000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml
Secondary Goat anti-rabbit IgG (H+L) at 1/10000 dilution
Predicted band size : 74 kDa Observed band size : 74 kDa
References for Anti-Arginyl tRNA synthetase antibody (ab31537)
This product has been referenced in:
Zhang X et al. Mutations in QARS, encoding glutaminyl-tRNA synthetase, cause progressive microcephaly, cerebral-cerebellar atrophy, and intractable seizures. Am J Hum Genet94:547-58 (2014).
Read more (PubMed: 24656866) »
Gulati P et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A110:2557-62 (2013).
Read more (PubMed: 23359686) »