Overview

  • Product nameAnti-Argonaute-2 antibody
    See all Argonaute-2 primary antibodies
  • Description
    Rabbit polyclonal to Argonaute-2
  • Tested applicationsSuitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Drosophila melanogaster
    Does not react with: Human
  • Immunogen

    Synthetic peptide corresponding to Drosophila melanogaster Argonaute-2 aa 550-650 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab24177)

  • Positive control
    • This antibody gave a positive result in WB on Drosophila lysate.
  • General notesPlease note the immunogen for this antibody has no sequence alignment to human dIFC2. When tested in human, non-specific binding is observed.

Properties

Applications

Our Abpromise guarantee covers the use of ab5072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/500 - 1/1000. Detects a band of approximately 55, 95 kDa (predicted molecular weight: 106 kDa).

Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Target

  • FunctionRequired for RNA-mediated gene silencing (RNAi) by the RNA-induced silencing complex (RISC). The 'minimal RISC' appears to include EIF2C2/AGO2 bound to a short guide RNA such as a microRNA (miRNA) or short interfering RNA (siRNA). These guide RNAs direct RISC to complementary mRNAs that are targets for RISC-mediated gene silencing. The precise mechanism of gene silencing depends on the degree of complementarity between the miRNA or siRNA and its target. Binding of RISC to a perfectly complementary mRNA generally results in silencing due to endonucleolytic cleavage of the mRNA specifically by EIF2C2/AGO2. Binding of RISC to a partially complementary mRNA results in silencing through inhibition of translation, and this is independent of endonuclease activity. May inhibit translation initiation by binding to the 7-methylguanosine cap, thereby preventing the recruitment of the translation initiation factor eIF4-E. May also inhibit translation initiation via interaction with EIF6, which itself binds to the 60S ribosomal subunit and prevents its association with the 40S ribosomal subunit. The inhibition of translational initiation leads to the accumulation of the affected mRNA in cytoplasmic processing bodies (P-bodies), where mRNA degradation may subsequently occur. In some cases RISC-mediated translational repression is also observed for miRNAs that perfectly match the 3' untranslated region (3'-UTR). Can also upregulate the translation of specific mRNAs under certain growth conditions. Binds to the AU element of the 3'-UTR of the TNF (TNF-alpha) mRNA and upregulates translation under conditions of serum starvation. Also required for transcriptional gene silencing (TGS), in which short RNAs known as antigene RNAs or agRNAs direct the transcriptional repression of complementary promoter regions.
  • Sequence similaritiesBelongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • DomainThe Piwi domain may perform RNA cleavage by a mechanism similar to that of RNase H. However while RNase H utilizes a triad of Asp-Asp-Glu (DDE) for metal ion coordination, this protein appears to utilize a triad of Asp-Asp-His (DDH).
  • Post-translational
    modifications
    Hydroxylated. 4-hydroxylation appears to enhance protein stability but is not required for miRNA-binding or endonuclease activity.
  • Cellular localizationCytoplasm > P-body. Nucleus. Translational repression of mRNAs results in their recruitment to P-bodies. Translocation to the nucleus requires IMP8.
  • Information by UniProt
  • Database links
    • Alternative names
      • Ago 2 antibody
      • AGO2_HUMAN antibody
      • Argonaute 2 antibody
      • argonaute 2, RISC catalytic component antibody
      • Argonaute RISC catalytic component 2 antibody
      • Argonaute2 antibody
      • CTA-204B4.6 antibody
      • dAgo2 antibody
      • eIF 2C 2 antibody
      • eIF-2C 2 antibody
      • eIF2C 2 antibody
      • Eif2c2 antibody
      • Eukaryotic translation initiation factor 2C 2 antibody
      • Eukaryotic translation initiation factor 2C subunit 2 antibody
      • hAgo2 antibody
      • MGC3183 antibody
      • PAZ Piwi domain protein antibody
      • PPD antibody
      • Protein argonaute-2 antibody
      • Protein slicer antibody
      • Q10 antibody
      • Slicer protein antibody
      see all

    Anti-Argonaute-2 antibody images

    • ab5072 staining Ago2 / eIF2C2 (green) in fruit fly (Drosophilia melanogaster) OSS cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Tween and blocked with 1% BSA for 1 hour at 22°C. Samples were incubated with primary antibody (1/200) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated donkey anti-rabbit IgG monoclonal (1/5000) was used as the secondary antibody.

      ab24609 was used to stain Nuclear Pore Complex Proteins (red).

      See Abreview

    • Anti-Argonaute-2 antibody (ab5072) at 1/500 dilution + Drosophila lysate at 20 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab6721)

      Predicted band size : 106 kDa
      Observed band size : 55,95 kDa (why is the actual band size different from the predicted?)

      Rabbit polyclonal to Ago2 (ab5072) at 1/500 on Drosophila lysate (20ug).

      Detects a band of approximately 55 + 95 kDa (predicted molecular weight: 106 kDa). Swissprot suggests Ago 2 may have 50kDa form.

      Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.

      Swissprot suggests Ago 2 may have 50kDa form.

    References for Anti-Argonaute-2 antibody (ab5072)

    This product has been referenced in:
    • Androsavich JR  et al. Disease-linked microRNA-21 exhibits drastically reduced mRNA binding and silencing activity in healthy mouse liver. RNA 18:1510-26 (2012). Read more (PubMed: 22740638) »
    • Schnettler E  et al. Noncoding flavivirus RNA displays RNA interference suppressor activity in insect and Mammalian cells. J Virol 86:13486-500 (2012). WB . Read more (PubMed: 23035235) »

    See all 6 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
    Sample Fruit fly (Drosophila melanogaster) Cell (OSS cells)
    Specification OSS cells
    Permeabilization Yes - Tween 0,2%
    Fixative Formaldehyde
    Username

    Ms. Nathalie GReD Gueguen

    Verified customer

    Submitted Sep 15 2014

    Unfortunately, we do not have any antibodies against Anti-ElF2C2 (hAgo2) which are directly conjugated to a fluorochrome available in our catalog.

    We do however have kits which can be used to conjugate your primary antibody with a fluorochrom...

    Read More

    Thank you very much for your interest in ab5072.

    To our knowledge, ab5072 has not been tested in IP or in a pull down assay.

    We do not use pull down assay as a application on our datasheets. But an antibody that is suitable for IP ...

    Read More

    Thank you for your enquiry. I sincerely apologize for the error. We just received information regarding the immunogen sequence for this antibody. On investigation by our laboratory, it seems that it does not have any alignment to the human protein....

    Read More
    Abcam has not validated the combination of species/application used in this Abreview.
    Application Immunoprecipitation
    Sample Fruit fly (Drosophila melanogaster) Cell lysate - whole cell (Drosophila S2R+ cells)
    Total protein in input 50 µg
    Specification Drosophila S2R+ cells
    Immuno-precipitation step Protein A
    Username

    Abcam user community

    Verified customer

    Submitted Oct 31 2006

    Thank you for your enquiry. Assuming that you are interested in an antibody to control for non-specific binding I would like to recommend that you use an affinity purified rabbit polyclonal antibody against an irrelevant target such as one of the e...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"