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Synthetic peptide corresponding to Argonaute-2 aa 350-450 conjugated to keyhole limpet haemocyanin.
(Peptide available as
Our Abpromise guarantee covers the use of ab32381 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 24280866|
|IHC-P||Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|ChIP||Use at an assay dependent concentration. PubMed: 19696410|
|RIP||Use at an assay dependent concentration.|
|WB||Use a concentration of 2 µg/ml. Detects a band of approximately 87 kDa (predicted molecular weight: 97 kDa).Can be blocked with Human Argonaute-2 peptide (ab32380).|
The identification of the 55 kDa band is unclear but this band has also been observed in Hela lysates in the following publication; http://www.ibiosci.or.kr/home/data/2004/0080213.pdf and in the Western Blot of ab5072, targeting Drosophila Ago2 / eIF2C2.
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