Overview

  • Product name
    Anti-Argonaute 3 / eIF2C 3 antibody
    See all Argonaute 3 / eIF2C 3 primary antibodies
  • Description
    Rabbit polyclonal to Argonaute 3 / eIF2C 3
  • Tested applications
    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat
    Predicted to work with: Human
  • Immunogen

    Synthetic peptide derived from within residues 350 - 450 of Human Argonaute 3.

    (Peptide available as ab24437.)

  • Positive control
    • This antibody gave a positive signal in rat and mouse dorsal root ganglia tissue lysates in WB.
  • General notes
    Protein: NP_079128 (human isoform a); NP_803171 (human isoform b); NP_700451 (mouse).

Properties

Applications

Our Abpromise guarantee covers the use of ab3593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
ICC/IF 1/200.
IHC-P Use at an assay dependent concentration.

Target

  • Function
    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs.
  • Sequence similarities
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localization
    Cytoplasm > P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730550L01Rik antibody
    • Ago 3 antibody
    • Ago3 antibody
    • AGO3_HUMAN antibody
    • argonaute 3 antibody
    • Argonaute3 antibody
    • eIF 2C 3 antibody
    • eIF-2C 3 antibody
    • eIF2C 3 antibody
    • Eif2c3 antibody
    • EIF2C3 protein antibody
    • Eukaryotic translation initiation factor 2C 3 antibody
    • Eukaryotic translation initiation factor 2C3 antibody
    • FLJ12765 antibody
    • hAgo3 antibody
    • MGC86946 antibody
    • Protein argonaute-3 antibody
    see all

Anti-Argonaute 3 / eIF2C 3 antibody images

  • Rat dorsal root ganglia were fixed in paraformaldehyde, blocked with 2% BSA for 30 minutes then incubated with ab3593 (1/200 dilution). For further details please refer to Hengst U et al  (2006).

  • All lanes : Anti-Argonaute 3 / eIF2C 3 antibody (ab3593) at 2 µg/ml

    Lane 1 : Dorsal Root Ganglia (Rat) Tissue Lysate
    Lane 2 : Dorsal Root Ganglia (Mouse) Tissue Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
    Additional bands at : 60 kDa. We are unsure as to the identity of these extra bands.
  • ab3593 staining Argonaute 3 / eIF2C 3 in murine testis tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
    Tissue was fixed with formaldehyde and an enzymatic antigen retrieval step was performed. Samples were then blocked with 10% serum for 30 minutes at 25°C and then incubated with ab3593 at a 1/50 dilution for 1 hour 30 minutes at 37°C. The secondary used was a biotin conjugated goat anti-rabbit polyclonal used at a 1/200 dilution.

    See Abreview

References for Anti-Argonaute 3 / eIF2C 3 antibody (ab3593)

This product has been referenced in:

See 1 Publication for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A dynabeads
Username

Abcam user community

Verified customer

Submitted Mar 21 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Formaldehyde
Antigen retrieval step
Enzymatic
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Dr. Lynn Dong

Verified customer

Submitted Feb 28 2012

Application
Western blot
Sample
Human Cell lysate - whole cell (human umbilical vein endothelial cells)
Loading amount
50 µg
Specification
human umbilical vein endothelial cells
Gel Running Conditions
Reduced Denaturing (SDS-PAGE 8%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 31 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (dissociated dorsal root ganglia)
Specification
dissociated dorsal root ganglia
Fixative
Paraformaldehyde
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2%
Username

Abcam user community

Verified customer

Submitted Jun 07 2006

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Rat Tissue lysate - whole (dorsal root ganglia explants)
Loading amount
50 µg
Specification
dorsal root ganglia explants
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

Submitted Apr 28 2006

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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