Recombinant Anti-AGO3 antibody [EPR9576] (ab154844)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9576] to AGO3
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-AGO3 antibody [EPR9576]
See all AGO3 primary antibodies -
Description
Rabbit monoclonal [EPR9576] to AGO3 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa, Daudi, A549 cell lysates. Human fetal brain and fetal kidney lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR9576 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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KO cell lines
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KO cell lysates
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154844 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/1000 - 1/10000. Predicted molecular weight: 97 kDa.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 97 kDa. |
Target
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Function
Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. -
Sequence similarities
Belongs to the argonaute family. Ago subfamily.
Contains 1 PAZ domain.
Contains 1 Piwi domain. -
Cellular localization
Cytoplasm > P-body. - Information by UniProt
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Database links
- Entrez Gene: 192669 Human
- Entrez Gene: 214150 Mouse
- Entrez Gene: 313593 Rat
- GenBank: NP_079128 Human
- GenBank: NP_803171 Human
- GenBank: NP_700451 Mouse
- Omim: 607355 Human
- SwissProt: Q9H9G7 Human
see all -
Alternative names
- 5730550L01Rik antibody
- Ago 3 antibody
- Ago3 antibody
see all
Images
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All lanes : Anti-AGO3 antibody [EPR9576] (ab154844) at 1/2000 dilution
Lane 1 : Wild-type HeLa lysate
Lane 2 : Argonaute RISC Catalytic Component 3 knockout HeLa lysate
Lane 3 : Daudi lysate
Lane 4 : A549 lysate
Lysates/proteins at 40 µg per lane.
Performed under reducing conditions.
Predicted band size: 97 kDaLanes 1-4: Merged signal (red and green). Green - ab154844 observed at 97 kDa. Red - loading control ab8245 observed at 37 kDa.
ab154844 Anti-AGO3 antibody [EPR9576] was shown to specifically react with Argonaute RISC Catalytic Component 3 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265320 (knockout cell lysate ab257819) was used. Wild-type and Argonaute RISC Catalytic Component 3 knockout samples were subjected to SDS-PAGE. ab154844 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 2000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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All lanes : Anti-AGO3 antibody [EPR9576] (ab154844) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal kidney lysate
Lysates/proteins at 10 µl/ml buffer per lane.
Predicted band size: 97 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab154844 has been referenced in 1 publication.
- Voutila J et al. Development and Mechanism of Small Activating RNA Targeting CEBPA, a Novel Therapeutic in Clinical Trials for Liver Cancer. Mol Ther 25:2705-2714 (2017). PubMed: 28882451