Overview

  • Product name
    Anti-Argonaute 4 antibody
  • Description
    Rabbit polyclonal to Argonaute 4
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A 15 amino acid synthetic peptide (Human) from near the N-terminus

  • Positive control
    • HeLa cell lysate

Properties

  • Form
    Lyophilised:Reconstitute with 200ul distilled sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
  • Storage instructions
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85077 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/1000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
IHC-P 1/200.

Target

  • Function
    Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Lacks endonuclease activity and does not appear to cleave target mRNAs. Also required for RNA-directed transcription and replication of the human hapatitis delta virus (HDV).
  • Sequence similarities
    Belongs to the argonaute family. Ago subfamily.
    Contains 1 PAZ domain.
    Contains 1 Piwi domain.
  • Cellular localization
    Cytoplasm > P-body.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5730550L01Rik antibody
    • Ago 4 antibody
    • Ago4 antibody
    • AGO4_HUMAN antibody
    • Argonaute4 antibody
    • EIF 2C 4 antibody
    • eIF-2C 4 antibody
    • eIF2C 4 antibody
    • eif2c4 antibody
    • Eukaryotic translation initiation factor 2C 4 antibody
    • eukaryotic translation initiation factor 2C antibody
    • hAgo4 antibody
    • KIAA1567 antibody
    • Piwi/Argonaute family protein meIF2C4 antibody
    • Protein argonaute-4 antibody
    see all

Anti-Argonaute 4 antibody images

  • Anti-Argonaute 4 antibody (ab85077) at 1/500 dilution + HeLa cell lysate

    Predicted band size : 97 kDa
    Observed band size : 97 kDa
  • ab85077 staining Argonaute 4 in Mouse testis tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 10% serum for 30 minutes at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/200 in PBS + casein) for 1 hour 30 mins at 37°C. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.

    See Abreview

References for Anti-Argonaute 4 antibody (ab85077)

This product has been referenced in:
  • Zhong F  et al. A SnoRNA-derived piRNA interacts with human interleukin-4 pre-mRNA and induces its decay in nuclear exosomes. Nucleic Acids Res 43:10474-91 (2015). WB . Read more (PubMed: 26405199) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (testis)
Specification
testis
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 11 2012

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application
CLIP
Sample
Human Cell lysate - whole cell (HEK 293T)
Specification
HEK 293T
Type
iCLIP
Irradiation Energy Level (mJ/cm2): 150
Wavelength (nm): 254
Positive control
Anti-hnRNP C antibody (sc-15386) bound protein A dynabeads, 0.1 mg/ml (antibody/beads)
Negative control
Protein A dynabeads without antibody bound
Immuno-precipitation step
Other - Protein A dynabeads
Username

Abcam user community

Verified customer

Submitted Mar 21 2012

Thank you for your enquiry. To date, neither of our anti-AGO4 antibodies have been tested in IP. Both ab85077 and ab52724 have thusfar only been tested in western blotting. They may be useful for IP but we do not have any data to suggest it. ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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